› 4wj0

X-ray diffraction
2.85Å resolution

Structure of PH1245, a cas1 from Pyrococcus horikoshii

Released:
DOI: 10.2210/pdb4wj0/pdb
PDB 4wj0 coloured by chain and viewed from the front.
PDB 4wj0 coloured by chain and viewed from the side.
PDB 4wj0 coloured by chain and viewed from the top.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Petit P, Brown G, Savchenko A, Yakunin AF

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 37.82 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O58938 (Residues: 1-322; Coverage: 100%)
Gene names: PH1245, cas1
Sequence domains: CRISPR associated protein Cas1
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 125.89Å b: 58.672Å c: 90.147Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.282
Expression system: Escherichia coli BL21(DE3)

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