› 4wi0

X-ray diffraction
1.93Å resolution

Crystal structure of Coh3ScaB-XDoc_M2ScaA complex: A C-terminal interface mutant of type II Cohesin-X-Dockerin complex from Acetivibrio cellulolyticus

Released:
DOI: 10.2210/pdb4wi0/pdb
PDB entry 4wi0 coloured by chain, front view.
PDB entry 4wi0 coloured by chain, side view.
PDB entry 4wi0 coloured by chain, top view.
Source organism: Acetivibrio cellulolyticus
Entry authors: Alves VD, Cameron K, Najmudin SH, Fontes CMGA

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182161 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dockerin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 18.32 KDa
Source organism: Acetivibrio cellulolyticus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7WYN3 (Residues: 407-573; Coverage: 18%)
Gene name: scaB
Sequence domains: Cohesin domain
Structure domains: Immunoglobulin-like
Endoglucanase Chain: B
Molecule details ›
Chain: B
Length: 156 amino acids
Theoretical weight: 16.49 KDa
Source organism: Acetivibrio cellulolyticus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9RPL0 (Residues: 1760-1915; Coverage: 8%)
Gene name: cipV
Sequence domains: CarboxypepD_reg-like domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 38.54Å b: 88.3Å c: 92.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.241
Expression system: Escherichia coli BL21

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