› 4whx

X-ray diffraction
2.05Å resolution

X-ray Crystal Structure of an Amino Acid Aminotransferase from Burkholderia pseudomallei Bound to the Co-factor Pyridoxal Phosphate

Released:
DOI: 10.2210/pdb4whx/pdb
PDB entry 4whx coloured by chain, front view.
PDB entry 4whx coloured by chain, side view.
PDB entry 4whx coloured by chain, top view.
Source organism: Burkholderia pseudomallei 576
Entry authors: Seattle Structural Genomics Center for Infectious Disease (SSGCID), Fairman JW, Dranow DM, Taylor BM, Lorimer D, Edwards TE

Function and Biology Details

Reaction catalysed: 
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101184 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Branched-chain-amino-acid aminotransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 311 amino acids
Theoretical weight: 34.56 KDa
Source organism: Burkholderia pseudomallei 576
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0E1U3Z8 (Residues: 1-307; Coverage: 100%)
Gene names: BUC_1039, ilvE
Sequence domains: Amino-transferase class IV
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ALA 4 x ALA
Ligand EDO 2 x EDO
1 modified residue:
Ligand LLP 6 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 104.14Å b: 230.35Å c: 77.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.198
Expression system: Escherichia coli BL21(DE3)

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