X-ray diffraction
2Å resolution

Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase SMc02828 (SmGhrA) from Sinorhizobium meliloti in complex with NADP and sulfate


Function and Biology Details

Reaction catalysed:
Glycolate + NADP(+) = glyoxylate + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2-Hacid_dh_C domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 37.26 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q92T34 (Residues: 1-319; Coverage: 100%)
Gene name: SMc02828
Sequence domains: D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

Cofactor: Ligand NAP 1 x NAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 108.153Å b: 108.153Å c: 80.657Å
α: 90° β: 90° γ: 120°
R R work R free
0.156 0.154 0.196
Expression system: Escherichia coli BL21(DE3)