› 4wcn

X-ray diffraction
1.75Å resolution

Crystal Structure of Tripeptide bound Cell Shape Determinant Csd4 protein from Helicobacter pylori

Released:
DOI: 10.2210/pdb4wcn/pdb
PDB 4wcn coloured by chain and viewed from the front.
PDB 4wcn coloured by chain and viewed from the side.
PDB 4wcn coloured by chain and viewed from the top.
Source organism: Helicobacter pylori G27
Primary publication:
Helical Shape of Helicobacter pylori Requires an Atypical Glutamine as a Zinc Ligand in the Carboxypeptidase Csd4.
Chan AC, Blair KM, Liu Y, Frirdich E, Gaynor EC, Tanner ME, Salama NR, Murphy ME
J. Biol. Chem. (2014)
PMID: 25505267

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Conserved hypothetical secreted protein Chain: A
Molecule details ›
Chain: A
Length: 439 amino acids
Theoretical weight: 50.18 KDa
Source organism: Helicobacter pylori G27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B5ZAD9 (Residues: 23-438; Coverage: 95%)
Gene name: HPG27_353
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand IOD 41 x IOD
Ligand ZN 1 x ZN
Ligand NA 2 x NA
Ligand 3KS 1 x 3KS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P212121
Unit cell:
a: 53.23Å b: 66.83Å c: 145.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.208
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Phosphinic Peptides as Tool Compounds for the Study of Pharmacologically Relevant Zn-Metalloproteases.
Georgiadis et al. (2022)
3 more

2 mentions without citation

Helical shape of Helicobacter pylori requires an atypical glutamine as a zinc ligand in the carboxypeptidase Csd4.
Chan et al. (2015)
1 more