› 4wav

X-ray diffraction
2.8Å resolution

Crystal Structure of Haloquadratum walsbyi bacteriorhodopsin mutant D93N

Released:
DOI: 10.2210/pdb4wav/pdb
PDB 4wav coloured by chain and viewed from the front.
PDB 4wav coloured by chain and viewed from the side.
PDB 4wav coloured by chain and viewed from the top.
Source organism: Haloquadratum walsbyi C23
Entry authors: Wang AHJ, Hsu MF, Yang CS, Fu HY

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin-I Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 28.51 KDa
Source organism: Haloquadratum walsbyi C23
Expression system: Escherichia coli
UniProt:
  • Canonical: G0LFX8 (Residues: 1-254; Coverage: 100%)
Gene names: Hqrw_1016, bop1
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

2 bound ligands:
Ligand RET 2 x RET
Ligand MPG 2 x MPG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: C2221
Unit cell:
a: 29.86Å b: 131.942Å c: 235.423Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.239
Expression system: Escherichia coli

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