4w9t

X-ray diffraction
1.57Å resolution

Function and Biology Details

Reactions catalysed:
1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-110142 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosyl isomerase A Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 25.33 KDa
Source organism: Streptomyces sp. Mg1
Expression system: Escherichia coli
UniProt:
  • Canonical: B4V386 (Residues: 1-243; Coverage: 100%)
Gene names: M444_10495, hisA, priA
Sequence domains: Histidine biosynthesis protein
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P43212
Unit cell:
a: 69.332Å b: 69.332Å c: 126.934Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.184
Expression system: Escherichia coli