X-ray diffraction
1.92Å resolution

Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with glucose


Function and Biology Details

Reaction catalysed:
Xyloglucan + H(2)O = xyloglucan oligosaccharides
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Glycoside hydrolase family 5 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 38.12 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli
  • Canonical: D2K7Z0 (Residues: 92-430; Coverage: 83%)
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P31
Unit cell:
a: 97.604Å b: 97.604Å c: 96.241Å
α: 90° β: 90° γ: 120°
R R work R free
0.181 0.179 0.216
Expression system: Escherichia coli