X-ray diffraction
2.9Å resolution

Crystal Structure of Full-Length Split GFP Mutant E124H/K126H With Copper Mediated Crystal Contacts, C 2 2 21 Space Group

Source organism: synthetic construct
Entry authors: Leibly DJ, Waldo GS, Yeates TO

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
fluorescent protein E124H/K126H Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 26.07 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 68.2Å b: 69.75Å c: 82.57Å
α: 90° β: 90° γ: 90°
R R work R free
0.271 0.264 0.332
Expression system: Escherichia coli