› 4w7f

X-ray diffraction
2.9Å resolution

Crystal Structure of Full-Length Split GFP Mutant E124H/K126H With Copper Mediated Crystal Contacts, C 2 2 21 Space Group

Released:
DOI: 10.2210/pdb4w7f/pdb
PDB 4w7f coloured by chain and viewed from the front.
PDB 4w7f coloured by chain and viewed from the side.
PDB 4w7f coloured by chain and viewed from the top.
Source organism: synthetic construct
Entry authors: Leibly DJ, Waldo GS, Yeates TO

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
fluorescent protein E124H/K126H Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 26.07 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand CU 1 x CU
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 68.2Å b: 69.75Å c: 82.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.264 0.332
Expression system: Escherichia coli

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Citations

3 review citations

Development of imaging scaffolds for cryo-electron microscopy.
Yeates et al. (2020)
2 more

1 mention without citation

A Suite of Engineered GFP Molecules for Oligomeric Scaffolding.
Leibly et al. (2015)