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A Suite of Engineered GFP Molecules for Oligomeric Scaffolding.

Leibly DJ, Arbing MA, Pashkov I, DeVore N, Waldo GS, Terwilliger TC, Yeates TO
Structure 23 1754-1768 (2015)

Cited: 13 times
EuropePMC logo PMID: 26278175

Abstract

Applications ranging from synthetic biology to protein crystallization could be advanced by facile systems for connecting multiple proteins together in predefined spatial relationships. One approach to this goal is to engineer many distinct assembly forms of a single carrier protein or scaffold, to which other proteins of interest can then be readily attached. In this work we chose GFP as a scaffold and engineered many alternative oligomeric forms, driven by either specific disulfide bond formation or metal ion addition. We generated a wide range of spatial arrangements of GFP subunits from 11 different oligomeric variants, and determined their X-ray structures in a total of 33 distinct crystal forms. Some of the oligomeric GFP variants show geometric polymorphism depending on conditions, while others show considerable geometric rigidity. Potential future applications of this system are discussed.

Articles - 4w76 mentioned but not cited (1)

  1. A Suite of Engineered GFP Molecules for Oligomeric Scaffolding. Leibly DJ, Arbing MA, Pashkov I, DeVore N, Waldo GS, Terwilliger TC, Yeates TO. Structure 23 1754-1768 (2015)


Reviews citing this publication (4)

  1. Split Green Fluorescent Proteins: Scope, Limitations, and Outlook. Romei MG, Boxer SG. Annu Rev Biophys 48 19-44 (2019)
  2. The design of symmetric protein nanomaterials comes of age in theory and practice. Yeates TO, Liu Y, Laniado J. Curr Opin Struct Biol 39 134-143 (2016)
  3. Better together: building protein oligomers naturally and by design. Gwyther REA, Jones DD, Worthy HL. Biochem Soc Trans 47 1773-1780 (2019)
  4. Development of imaging scaffolds for cryo-electron microscopy. Yeates TO, Agdanowski MP, Liu Y. Curr Opin Struct Biol 60 142-149 (2020)

Articles citing this publication (8)

  1. Rewiring T-cell responses to soluble factors with chimeric antigen receptors. Chang ZL, Lorenzini MH, Chen X, Tran U, Bangayan NJ, Chen YY. Nat Chem Biol 14 317-324 (2018)
  2. A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold. Liu Y, Huynh DT, Yeates TO. Nat Commun 10 1864 (2019)
  3. Characterization of Split Fluorescent Protein Variants and Quantitative Analyses of Their Self-Assembly Process. Köker T, Fernandez A, Pinaud F. Sci Rep 8 5344 (2018)
  4. Synthetic cytokine receptors transmit biological signals using artificial ligands. Engelowski E, Schneider A, Franke M, Xu H, Clemen R, Lang A, Baran P, Binsch C, Knebel B, Al-Hasani H, Moll JM, Floß DM, Lang PA, Scheller J. Nat Commun 9 2034 (2018)
  5. Construction of supramolecular nanotubes from protein crystals. Nguyen TK, Negishi H, Abe S, Ueno T. Chem Sci 10 1046-1051 (2019)
  6. DNA-Directed Protein Packing within Single Crystals. Winegar PH, Hayes OG, McMillan JR, Figg CA, Focia PJ, Mirkin CA. Chem 6 1007-1017 (2020)
  7. Co-crystallization with diabodies: A case study for the introduction of synthetic symmetry. Chesterman C, Arnold E. Structure 29 598-605.e3 (2021)
  8. Designed Artificial Protein Heterodimers With Coupled Functions Constructed Using Bio-Orthogonal Chemistry. Johnson RL, Blaber HG, Evans T, Worthy HL, Pope JR, Jones DD. Front Chem 9 733550 (2021)


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