X-ray diffraction
2.1Å resolution

Crystal Structure of Full-Length Split GFP Mutant E115C/T118H With Metal Mediated Crystal Contacts, P 1 21 1 Space Group


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
fluorescent protein E115C/T118H Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 229 amino acids
Theoretical weight: 26.07 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 67.44Å b: 119.79Å c: 130.57Å
α: 90° β: 89.99° γ: 90°
R R work R free
0.214 0.212 0.235
Expression system: Escherichia coli