4w73

X-ray diffraction
2.79Å resolution

Crystal Structure of Full-Length Split GFP Mutant E115C/T118H Disulfide Dimer P 21 21 21

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
fluorescent protein E115C/T118H Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.07 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 69.74Å b: 70.58Å c: 77.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.221 0.297
Expression system: Escherichia coli