› 4w72

X-ray diffraction
2Å resolution

Crystal Structure of Full-Length Split GFP Mutant E115C/T118H Disulfide Dimer With Copper Mediated Crystal Contacts, P 21 21 21, Form 1

Released:
DOI: 10.2210/pdb4w72/pdb
PDB 4w72 coloured by chain and viewed from the front.
PDB 4w72 coloured by chain and viewed from the side.
PDB 4w72 coloured by chain and viewed from the top.
Source organism: synthetic construct
Primary publication:
A Suite of Engineered GFP Molecules for Oligomeric Scaffolding.
Leibly DJ, Arbing MA, Pashkov I, DeVore N, Waldo GS, Terwilliger TC, Yeates TO
Structure 23 1754-68 (2015)
PMID: 26278175

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
fluorescent protein E115C/T118H Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.07 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand CU 1 x CU
1 modified residue:
Ligand CRO 2 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 72.54Å b: 74.4Å c: 89.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.19 0.235
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Development of imaging scaffolds for cryo-electron microscopy.
Yeates et al. (2020)
2 more

1 mention without citation

A Suite of Engineered GFP Molecules for Oligomeric Scaffolding.
Leibly et al. (2015)