› 4w6t

X-ray diffraction
1.6Å resolution

Crystal Structure of Full-Length Split GFP Mutant E115H/T118H With Copper Mediated Crystal Contacts, P 43 21 2 Space Group

Released:
DOI: 10.2210/pdb4w6t/pdb
PDB 4w6t coloured by chain and viewed from the front.
PDB 4w6t coloured by chain and viewed from the side.
PDB 4w6t coloured by chain and viewed from the top.
Source organism: synthetic construct
Primary publication:
A Suite of Engineered GFP Molecules for Oligomeric Scaffolding.
Leibly DJ, Arbing MA, Pashkov I, DeVore N, Waldo GS, Terwilliger TC, Yeates TO
Structure 23 1754-68 (2015)
PMID: 26278175

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
fluorescent protein E115H/T118H Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 26.1 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand CU 2 x CU
Ligand EDO 11 x EDO
Ligand BR 1 x BR
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43212
Unit cell:
a: 105.3Å b: 105.3Å c: 69.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.207
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Development of imaging scaffolds for cryo-electron microscopy.
Yeates et al. (2020)
2 more

1 mention without citation

A Suite of Engineered GFP Molecules for Oligomeric Scaffolding.
Leibly et al. (2015)