› 4w6l

X-ray diffraction
2.45Å resolution

Crystal Structure of Full-Length Split GFP Mutant D117C Disulfide Dimer, I 41 2 2 Space Group

Released:
DOI: 10.2210/pdb4w6l/pdb
PDB 4w6l coloured by chain and viewed from the front.
PDB 4w6l coloured by chain and viewed from the side.
PDB 4w6l coloured by chain and viewed from the top.
Source organism: synthetic construct
Entry authors: Leibly DJ, Waldo GS, Yeates TO

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
fluorescent protein D117C Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 26.82 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I4122
Unit cell:
a: 108.43Å b: 108.43Å c: 101.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.252 0.278
Expression system: Escherichia coli

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Citations

3 review citations

Development of imaging scaffolds for cryo-electron microscopy.
Yeates et al. (2020)
2 more

1 mention without citation

A Suite of Engineered GFP Molecules for Oligomeric Scaffolding.
Leibly et al. (2015)