4v9n › Citations

Crystal structure of the 70S ribosome bound with the Q253P mutant form of release factor RF2.

Santos N, Zhu J, Donohue JP, Korostelev AA, Noller HF
Structure 21 1258-63 (2013)
Cited: 19 times
EuropePMC logo PMID: 23769667

Abstract

Bacterial translation termination is mediated by release factors RF1 and RF2, which recognize stop codons and catalyze hydrolysis of the peptidyl-tRNA ester bond. The catalytic mechanism has been debated. We proposed that the backbone amide NH group, rather than the side chain, of the glutamine of the universally conserved GGQ motif participates in catalysis by H-bonding to the tetrahedral transition-state intermediate and by product stabilization. This was supported by complete loss of RF1 catalytic activity when glutamine is replaced by proline, the only residue that lacks a backbone NH group. Here, we present the 3.4 Å crystal structure of the ribosome complex containing the RF2 Q253P mutant and find that its fold, including the GGP sequence, is virtually identical to that of wild-type RF2. This rules out proline-induced misfolding and further supports the proposal that catalytic activity requires interaction of the Gln-253 backbone amide with the 3' end of peptidyl-tRNA.

Articles - 4v9n mentioned but not cited (1)

  1. Inhibition of translation termination by small molecules targeting ribosomal release factors. Ge X, Oliveira A, Hjort K, Bergfors T, Gutiérrez-de-Terán H, Andersson DI, Sanyal S, Åqvist J. Sci Rep 9 15424 (2019)


Reviews citing this publication (5)

  1. Translation in Prokaryotes. Rodnina MV. Cold Spring Harb Perspect Biol 10 a032664 (2018)
  2. Therapeutic suppression of premature termination codons: mechanisms and clinical considerations (review). Karijolich J, Yu YT. Int J Mol Med 34 355-362 (2014)
  3. Ribosome Rescue Pathways in Bacteria. Müller C, Crowe-McAuliffe C, Wilson DN. Front Microbiol 12 652980 (2021)
  4. Life under the Microscope: Single-Molecule Fluorescence Highlights the RNA World. Ray S, Widom JR, Walter NG. Chem Rev 118 4120-4155 (2018)
  5. Diversity and Similarity of Termination and Ribosome Rescue in Bacterial, Mitochondrial, and Cytoplasmic Translation. Korostelev AA. Biochemistry (Mosc) 86 1107-1121 (2021)

Articles citing this publication (13)

  1. Structure of the mammalian ribosomal pre-termination complex associated with eRF1.eRF3.GDPNP. des Georges A, Hashem Y, Unbehaun A, Grassucci RA, Taylor D, Hellen CU, Pestova TV, Frank J. Nucleic Acids Res 42 3409-3418 (2014)
  2. RNA Bricks--a database of RNA 3D motifs and their interactions. Chojnowski G, Walen T, Bujnicki JM. Nucleic Acids Res 42 D123-31 (2014)
  3. Uniformity of Peptide Release Is Maintained by Methylation of Release Factors. Pierson WE, Hoffer ED, Keedy HE, Simms CL, Dunham CM, Zaher HS. Cell Rep 17 11-18 (2016)
  4. On the pH dependence of class-1 RF-dependent termination of mRNA translation. Indrisiunaite G, Pavlov MY, Heurgué-Hamard V, Ehrenberg M. J Mol Biol 427 1848-1860 (2015)
  5. Mechanistic insights into the alternative translation termination by ArfA and RF2. Ma C, Kurita D, Li N, Chen Y, Himeno H, Gao N. Nature 541 550-553 (2017)
  6. Mechanism of ribosome rescue by ArfA and RF2. Demo G, Svidritskiy E, Madireddy R, Diaz-Avalos R, Grant T, Grigorieff N, Sousa D, Korostelev AA. Elife 6 e23687 (2017)
  7. The structural basis for release-factor activation during translation termination revealed by time-resolved cryogenic electron microscopy. Fu Z, Indrisiunaite G, Kaledhonkar S, Shah B, Sun M, Chen B, Grassucci RA, Ehrenberg M, Frank J. Nat Commun 10 2579 (2019)
  8. Structural Basis for Translation Termination on a Pseudouridylated Stop Codon. Svidritskiy E, Madireddy R, Korostelev AA. J Mol Biol 428 2228-2236 (2016)
  9. Atomic mutagenesis of stop codon nucleotides reveals the chemical prerequisites for release factor-mediated peptide release. Hoernes TP, Clementi N, Juen MA, Shi X, Faserl K, Willi J, Gasser C, Kreutz C, Joseph S, Lindner H, Hüttenhofer A, Erlacher MD. Proc Natl Acad Sci U S A 115 E382-E389 (2018)
  10. Extensive ribosome and RF2 rearrangements during translation termination. Svidritskiy E, Demo G, Loveland AB, Xu C, Korostelev AA. Elife 8 e46850 (2019)
  11. Peptide release promoted by methylated RF2 and ArfA in nonstop translation is achieved by an induced-fit mechanism. Zeng F, Jin H. RNA 22 49-60 (2016)
  12. Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis. Shimokawa-Chiba N, Müller C, Fujiwara K, Beckert B, Ito K, Wilson DN, Chiba S. Nat Commun 10 5397 (2019)
  13. Mechanism of Inhibition of Translation Termination by Blasticidin S. Svidritskiy E, Korostelev AA. J Mol Biol 430 591-593 (2018)


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