Structure analysis

Crystal structure of YfiA bound to the 70S ribosome.

X-ray diffraction
2.7Å resolution
Assembly composition:
hetero 53-mer (preferred)
Entry contents: 50 distinct polypeptide molecules
3 distinct RNA molecules

Assemblies

Assembly 1
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Multimeric state: hetero 53-mer
Accessible surface area: 733586.42 Å2
Buried surface area: 258486.46 Å2
Dissociation area: 892.15 Å2
Dissociation energy (ΔGdiss): 7.02 kcal/mol
Dissociation entropy (TΔSdiss): 13.56 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-142324
Assembly 2 (preferred)
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Multimeric state: hetero 53-mer
Accessible surface area: 728725.61 Å2
Buried surface area: 259419.0 Å2
Dissociation area: 884.64 Å2
Dissociation energy (ΔGdiss): 5.78 kcal/mol
Dissociation entropy (TΔSdiss): 13.56 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-142324

Macromolecules

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Chains: AJ, CJ
Length: 105 amino acids
Theoretical weight: 11.95 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHN7 (Residues: 1-105; Coverage: 100%)
Gene names: TTHA1693, rpsJ
Pfam: Ribosomal protein S10p/S20e
InterPro:

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Chains: AK, CK
Length: 129 amino acids
Theoretical weight: 13.74 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80376 (Residues: 1-129; Coverage: 100%)
Gene names: TTHA1666, rps11, rpsK
Pfam: Ribosomal protein S11
InterPro:

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Chains: AL, CL
Length: 132 amino acids
Theoretical weight: 14.64 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHN3 (Residues: 1-132; Coverage: 100%)
Gene names: TTHA1697, rpsL
Pfam: Ribosomal protein S12/S23
InterPro:

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Chains: AO, CO
Length: 89 amino acids
Theoretical weight: 10.58 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SJ76 (Residues: 1-89; Coverage: 100%)
Gene names: TTHA1138, rpsO
Pfam: Ribosomal protein S15
InterPro:

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Chains: AP, CP
Length: 88 amino acids
Theoretical weight: 10.41 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SJH3 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA1035, rpsP
Pfam: Ribosomal protein S16
InterPro:

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Chains: AR, CR
Length: 88 amino acids
Theoretical weight: 10.26 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SLQ0 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA0243, rpsR
Pfam: Ribosomal protein S18
InterPro:

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Chains: AS, CS
Length: 93 amino acids
Theoretical weight: 10.61 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHP2 (Residues: 1-93; Coverage: 100%)
Gene names: TTHA1688, rpsS
Pfam: Ribosomal protein S19
InterPro:

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Chains: AT, CT
Length: 106 amino acids
Theoretical weight: 11.74 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80380 (Residues: 1-106; Coverage: 100%)
Gene names: TTHA1397, rps20, rpsT
Pfam: Ribosomal protein S20
InterPro:

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Chains: AU, CU
Length: 27 amino acids
Theoretical weight: 3.35 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SIH3 (Residues: 1-27; Coverage: 100%)
Gene names: TTHA1396, rpsU
Pfam: 30S ribosomal protein Thx
InterPro:

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Chains: AY, CY
Length: 119 amino acids
Theoretical weight: 13.63 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AD49 (Residues: 1-113; Coverage: 100%)
Gene names: JW2578, b2597, raiA, yfiA
Pfam: Sigma 54 modulation protein / S30EA ribosomal protein
InterPro:

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Chains: BE, DE
Length: 206 amino acids
Theoretical weight: 22.45 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHN8 (Residues: 1-206; Coverage: 100%)
Gene names: TTHA1692, rplC
Pfam: Ribosomal protein L3
InterPro:

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Chains: BF, DF
Length: 210 amino acids
Theoretical weight: 23.27 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHN9 (Residues: 1-210; Coverage: 100%)
Gene names: TTHA1691, rplD
Pfam: Ribosomal protein L4/L1 family
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Chains: BN, DN
Length: 140 amino acids
Theoretical weight: 15.93 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P60488 (Residues: 1-140; Coverage: 100%)
Gene names: TTHA1465, rplM
Pfam: Ribosomal protein L13
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Chains: BO, DO
Length: 122 amino acids
Theoretical weight: 13.32 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHP8 (Residues: 1-122; Coverage: 100%)
Gene names: TTHA1682, rplN
Pfam: Ribosomal protein L14p/L23e
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Chains: BQ, DQ
Length: 141 amino acids
Theoretical weight: 15.99 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P60489 (Residues: 1-141; Coverage: 100%)
Gene names: TTHA1685, rplP
Pfam: Ribosomal protein L16p/L10e
InterPro:

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Chains: BR, DR
Length: 118 amino acids
Theoretical weight: 13.75 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q9Z9H5 (Residues: 1-118; Coverage: 100%)
Gene names: TTHA1663, rpl17, rplQ
Pfam: Ribosomal protein L17
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Chains: BS, DS
Length: 112 amino acids
Theoretical weight: 12.64 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHQ4 (Residues: 1-112; Coverage: 100%)
Gene names: TTHA1676, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
InterPro:

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Chains: BT, DT
Length: 146 amino acids
Theoretical weight: 17.19 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P60490 (Residues: 1-146; Coverage: 100%)
Gene names: TTHA1031, rplS
Pfam: Ribosomal protein L19
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Chains: BU, DU
Length: 118 amino acids
Theoretical weight: 13.78 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P60491 (Residues: 1-118; Coverage: 100%)
Gene names: TTHA0553, rplT
Pfam: Ribosomal protein L20
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Chains: BV, DV
Length: 101 amino acids
Theoretical weight: 11.07 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P60492 (Residues: 1-101; Coverage: 100%)
Gene names: TTHA1783, rplU
Pfam: Ribosomal prokaryotic L21 protein
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Chains: BX, DX
Length: 96 amino acids
Theoretical weight: 10.76 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHP0 (Residues: 1-96; Coverage: 100%)
Gene names: TTHA1690, rplW
Pfam: Ribosomal protein L23
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Chains: B0, D0
Length: 85 amino acids
Theoretical weight: 9.53 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P60493 (Residues: 1-85; Coverage: 100%)
Gene names: TTHA1782, rpmA
Pfam: Ribosomal L27 protein
InterPro:

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Chains: B1, D1
Length: 98 amino acids
Theoretical weight: 11 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P60494 (Residues: 1-98; Coverage: 100%)
Gene names: TTHA0042, rpmB
Pfam: Ribosomal L28 family
InterPro:

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Chains: B2, D2
Length: 72 amino acids
Theoretical weight: 8.67 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHP6 (Residues: 1-72; Coverage: 100%)
Gene names: TTHA1684, rpmC
Pfam: Ribosomal L29 protein
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Chains: B5, D5
Length: 60 amino acids
Theoretical weight: 6.72 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80339 (Residues: 1-60; Coverage: 100%)
Gene names: TTHA0418, rpl32, rpmF
Pfam: Ribosomal L32p protein family
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Chains: B7, D7
Length: 49 amino acids
Theoretical weight: 6.13 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80340 (Residues: 1-49; Coverage: 100%)
Gene names: TTHA0446, rpl34, rpmH
Pfam: Ribosomal protein L34
InterPro:

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Chains: B9, D9
Length: 37 amino acids
Theoretical weight: 4.44 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80256 (Residues: 1-37; Coverage: 100%)
Gene names: rpl36, rpmJ
Pfam: Ribosomal protein L36
InterPro:

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Name: 16S ribosomal RNA
Representative chains: AA, CA
Length: 1522 nucleotides
Theoretical weight: 493.96 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: 23S ribosomal RNA
Representative chains: BA, DA
Length: 2915 nucleotides
Theoretical weight: 947.9 KDa
Rfam: Bacterial large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: BB, DB
Length: 122 nucleotides
Theoretical weight: 39.49 KDa
Rfam: 5S ribosomal RNA

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