Protein Data Bank in Europe

Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val

(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate

(S)-ureidoglycolate = glyoxylate + urea

4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O

4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O

Hydrogen sulfide + 6 oxidized ferredoxin [iron-sulfur] cluster + 3 H(2)O = sulfite + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H(+)

Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate

(1a) cholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = (25S)-26-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O

5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO(2)

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH

ATP + adenosine = ADP + AMP

ATP + thymidine = ADP + thymidine 5'-phosphate

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate

ATP + thiamine = AMP + thiamine diphosphate

ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate

Creatinine + H(2)O = creatine

5-hydroxyisourate + H(2)O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate

Palmitoyl-CoA + H(2)O = CoA + palmitate

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol

N-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate

ATP-dependent breakage, passage and rejoining of double-stranded DNA

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.

Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.

Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-

Prephenate = phenylpyruvate + H(2)O + CO(2)

NTP + H(2)O = NDP + phosphate

(Polyphosphate)(n) + H(2)O = (polyphosphate)(n-1) + phosphate

1-haloalkane + H(2)O = a primary alcohol + halide

ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate

ATP + deamido-NAD(+) + L-glutamine + H(2)O = AMP + diphosphate + NAD(+) + L-glutamate

(1a) L-glutamine + H(2)O = L-glutamate + NH(3)

3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate

RX + glutathione = HX + R-S-glutathione

6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate

ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide

Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine

S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]

S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]

Acetyl-CoA + H(2)O + glyoxylate = (S)-malate + CoA

Arsenate + mycothiol = arseno-mycothiol + H(2)O

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O

4-fumarylacetoacetate + H(2)O = acetoacetate + fumarate

Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO(2) 

(1a) L-serine = 2-aminoprop-2-enoate + H(2)O

dUTP + H(2)O = dUMP + diphosphate

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate

ATP + a protein = ADP + a phosphoprotein

ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine

3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid

Chorismate = prephenate

NDP-glucose + 3-phospho-D-glycerate = NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate

[Biotin carboxyl-carrier protein]-N(6)-carboxybiotinyl-L-lysine + acetyl-CoA = [biotin carboxyl-carrier protein]-N(6)-biotinyl-L-lysine + malonyl-CoA

Aceneneuramate = N-acetyl-D-mannosamine + pyruvate

A beta-lactam + H(2)O = a substituted beta-amino acid

D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H(2)O

2 bilirubin + O(2) = 2 biliverdin + 2 H(2)O

ATP + UMP = ADP + UDP

L-aspartate 4-semialdehyde + phosphate + NADP(+) = L-4-aspartyl phosphate + NADPH

L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and similar substrates, releasing cellobiose from the reducing ends of the chains.

NAD(P)H + O(2) = NAD(P)(+) + H(2)O(2)

Succinate + a quinone = fumarate + a quinol

O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH = [CysO sulfur-carrier protein]-Gly-NH-CH(2)-C(O)-S-L-cysteine + phosphate

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.

ATP + H(2)O = ADP + phosphate

Release of N-terminal proline from a peptide.

10-formyltetrahydrofolate + H(2)O = formate + tetrahydrofolate

GTP + H(2)O = GDP + phosphate

Cleavage of peptide bonds with very broad specificity.

2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate

A phenyl acetate + H(2)O = a phenol + acetate

Triacylglycerol + H(2)O = diacylglycerol + a carboxylate

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins

A [lipoyl-carrier protein]-N(6)-((R)-dihydrolipoyl)-L-lysine + ROOH = a [lipoyl-carrier protein]-N(6)-((R)-lipoyl)-L-lysine + H(2)O + ROH

An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).

Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.

Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.

Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.

Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans

ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate

4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate

GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]

N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate

4-(2-carboxyphenyl)-4-oxobutanoyl-CoA = 1,4-dihydroxy-2-naphthoyl-CoA + H(2)O

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides

2 superoxide + 2 H(+) = O(2) + H(2)O(2)

NADPH + NAD(+) + H(+)(Side 1) = NADP(+) + H(+)(Side 2) + NADH

N-carbamoylputrescine + H(2)O = putrescine + CO(2) + NH(3)

2-(N(omega)-L-arginino)succinate = fumarate + L-arginine

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]

(+)-muconolactone = 5-oxo-4,5-dihydrofuran-2-acetate

5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+)

ATP + dTMP = ADP + dTDP

L-alanine + H(2)O + NAD(+) = pyruvate + NH(3) + NADH

An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH

ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate

Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H(2)O

ATP + 7,8-diaminononanoate + CO(2) = ADP + phosphate + dethiobiotin

2-carboxy-2,5-dihydro-5-oxofuran-2-acetate = cis,cis-butadiene-1,2,4-tricarboxylate

[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate

(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH

S-adenosyl-L-homocysteine + H(2)O = L-homocysteine + adenosine

Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate

Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine

AMP + H(2)O = D-ribose 5-phosphate + adenine

7,8-dihydroneopterin 3'-triphosphate + H(2)O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine

N-acetyl-O-acetylneuraminate + H(2)O = N-acetylneuraminate + acetate

DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine

(S)-lactate + NAD(+) = pyruvate + NADH

Diphosphate + H(2)O = 2 phosphate

L-lysine + NADPH + O(2) = N(6)-hydroxy-L-lysine + NADP(+) + H(2)O

Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH

(S)-dihydroorotate + fumarate = orotate + succinate

5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate

Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate

3-deoxy-D-manno-octulosonate 8-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate + phosphate

2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH

ATP = 3',5'-cyclic AMP + diphosphate

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO(2)

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids

S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid

(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2)

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H(2)O = tetrahydrofolate + 2-dehydropantoate

L-alanine = D-alanine

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate

Pyridoxamine 5'-phosphate + H(2)O + O(2) = pyridoxal 5'-phosphate + NH(3) + H(2)O(2)

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O

Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.

3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH(2) + O(2) = 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H(2)O

3-phospho-D-glycerate + NAD(+) = 3-phosphonooxypyruvate + NADH

TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.

ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine

(1a) [enzyme]-L-histidine + 2,3-bisphospho-D-glycerate = [enzyme]-N(tau)-phospho-L-histidine + 2/3-phospho-D-glycerate

2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + NADPH

1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + H(2)O = 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + acetate

Release of an N-terminal D-amino acid from a peptide, Xaa-|-Yaa-, in which Xaa is preferably D-Ala, D-Ser or D-Thr. D-amino acid amides and methyl esters also are hydrolyzed, as is glycine amide.

Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)

2-oxo-2H-pyran-4,6-dicarboxylate + H(2)O = (1E)-4-oxobut-1-ene-1,2,4-tricarboxylate

Phosphonoacetaldehyde + H(2)O = acetaldehyde + phosphate

Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products

Hydrolysis of the bonds linking certain hydrophobic residues in hemoglobin or globin. Also cleaves small molecules substrates such as Ala-Leu-Glu-Arg-Thr-Phe-|-Phe(NO(2))-Ser-Phe-Pro-Thr.

Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites

Isochorismate + H(2)O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate

D-arabinose 5-phosphate = D-ribulose 5-phosphate

(R)-2-hydroxyglutarate + NAD(+) = 2-oxoglutarate + NADH

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins

2 H(2)O(2) = O(2) + 2 H(2)O

(S)-malate = fumarate + H(2)O

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

ATP + D-ribose = ADP + D-ribose 5-phosphate

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin

S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate

(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine

ATP + L-phenylalanine + H(2)O = AMP + diphosphate + D-phenylalanine

5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine

An ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO(2) + a holo-[acyl-carrier protein]

L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH

Adenosine + H(2)O = inosine + NH(3)

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate

Alpha-D-glucose = beta-D-glucose

1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO(2) + H(2)O

Beta-nicotinate D-ribonucleotide + diphosphate + CO(2) = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate

2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate

ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)

N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA

2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O

N(6)-(Delta(2)-isopentenyl)-adenosine 5'-phosphate + H(2)O = N(6)-(dimethylallyl)adenine + D-ribose 5'-phosphate

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate

Beta-D-ribopyranose = beta-D-ribofuranose

ATP + glycerol = ADP + sn-glycerol 3-phosphate

(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine

L-lysine = cadaverine + CO(2)

UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose

(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine

ATP + shikimate = ADP + shikimate 3-phosphate

ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)

dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate

Peptidylproline (omega=180) = peptidylproline (omega=0)

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol

Protein L-glutamine + H(2)O = protein L-glutamate + NH(3)

Shikimate + NADP(+) = 3-dehydroshikimate + NADPH

5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH

ATP + (R)-pantothenate = ADP + (R)-4'-phosphopantothenate

NADH + ROOH + H(+) = NAD(+) + H(2)O + ROH

A 5'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate

2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O

Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.

Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans

H(2)CO(3) = CO(2) + H(2)O

Acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N(3)-acetyl-2-deoxystreptamine antibiotic

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides

4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate

Myo-inositol phosphate + H(2)O = myo-inositol + phosphate

L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate + NH(3) + NADPH

Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans

A phosphatidylcholine + H(2)O = 1,2-diacyl-sn-glycerol + phosphocholine

(3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H(2)O

A chalcone = a flavanone

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans

Protoheme + 3 reduced acceptor + 3 O(2) = mycobilin a + Fe(2+) + 3 acceptor + 3 H(2)O

ATP + H(2)O + H(+)(Side 1) + K(+)(Side 2) = ADP + phosphate + H(+)(Side 2) + K(+)(Side 1)

Succinyl-CoA + (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + H(2)O = CoA + N-succinyl-L-2-amino-6-oxoheptanedioate

2-iminobutanoate + H(2)O = 2-oxobutanoate + NH(3)

Succinyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-succinyldihydrolipoyl)lysine

Cyclo(L-tyrosyl-L-tyrosyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = mycocyclosin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H(2)O

Isocitrate = succinate + glyoxylate

2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate

Quercetin + O(2) = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H(+)

Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine

Choline = trimethylamine + acetaldehyde

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides

D-galactose + O(2) = D-galacto-hexodialdose + H(2)O(2)

(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)

Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH

(R)-10-hydroxystearate = oleate + H(2)O

(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate

An aldehyde + NAD(P)(+) + H(2)O = a carboxylate + NAD(P)H

N(6)-prenyladenine + acceptor + H(2)O = adenine + 3-methylbut-2-enal + reduced acceptor

(2S)-2-hydroxy-3,4-dioxopentyl phosphate = 3-hydroxy-2,4-dioxopentyl phosphate

Hydrolyzes poly(ADP-D-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-D-ribose

(1a) 2 cob(II)alamin + 2 [corrinoid adenosyltransferase] = 2 [corrinoid adenosyltransferase]-cob(II)alamin

(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate

(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + ditrans,polycis-polyprenyl diphosphate (n = 10-55)

Chorismate = isochorismate

L-arginine + 2-oxoglutarate + O(2) = (3S)-3-hydroxy-L-arginine + succinate + CO(2)

ATP + phenylacetate + CoA = AMP + diphosphate + phenylacetyl-CoA

3-oxoadipate + NAD(P)(+) = 2-maleylacetate + NAD(P)H

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains

Isochorismate = salicylate + pyruvate

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH

2 glutathione + ROOH = glutathione disulfide + H(2)O + ROH

Maltose = alpha,alpha-trehalose

Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.

5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H

6-phospho-D-glucono-1,5-lactone + H(2)O = 6-phospho-D-gluconate

D-xylopyranose = D-xylulose

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate

2 nitric oxide + 2 O(2) + NAD(P)H = 2 nitrate + NAD(P)(+) + H(+)

3-dehydroquinate = 3-dehydroshikimate + H(2)O

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)(+) + H(2)O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H

S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA 

Nucleoside 2',3'-cyclic phosphate + H(2)O = nucleoside 3'-phosphate

Nucleoside 3',5'-cyclic phosphate + H(2)O = nucleoside 5'-phosphate

S-adenosyl-L-methionine + pseudouridine(1915) in 23S rRNA = S-adenosyl-L-homocysteine + N(3)-methylpseudouridine(1915) in 23S rRNA

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units

Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate

4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO(2)

2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate

Succinate semialdehyde + NAD(P)(+) + H(2)O = succinate + NAD(P)H

1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide

ATP + pyruvate = ADP + phosphoenolpyruvate

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA

GTP = 3',5'-cyclic GMP + diphosphate

ADP-alpha-D-glucose + D-glucose 6-phosphate = ADP + alpha,alpha-trehalose 6-phosphate

Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-|-Xaa- and -Yaa-Xaa-Gly-Xaa-|-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid).

Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)

An ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO(2) + a holo-[acyl-carrier protein]

ATP + AMP = 2 ADP

Trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD = trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH(2)

L-histidinol phosphate + H(2)O = L-histidinol + phosphate

Pectin + n H(2)O = n methanol + pectate

Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate

Blasticidin S + H(2)O = deaminohydroxyblasticidin S + NH(3)

ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA

The primary cleavage site is at 67-His-|-Lys-68 in human C5a with a minor secondary cleavage site at 58-Ala-|-Ser-59

A phosphate monoester + H(2)O = an alcohol + phosphate

dCTP + 2 H(2)O = dUMP + diphosphate + NH(3)

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H(2)O = 1-alkyl-sn-glycero-3-phosphocholine + acetate

D-glucose 6-phosphate + oxidized coenzyme F420 = D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420

An alpha-L-fucoside + H(2)O = L-fucose + an alcohol

(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O

L-lysyl-tRNA(Lys) + phosphatidylglycerol = tRNA(Lys) + 3-O-L-lysyl-1-O-phosphatidylglycerol

GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose

ATP + NAD(+) = ADP + NADP(+)

L-asparagine + H(2)O = L-aspartate + NH(3)

S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine

2-phosphoglycolate + H(2)O = glycolate + phosphate

D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate

Acetyl-CoA + phosphate = CoA + acetyl phosphate

Acetyl-CoA + 3-methyl-2-oxobutanoate + H(2)O = (2S)-2-isopropylmalate + CoA

ATP + pyridoxal = ADP + pyridoxal 5'-phosphate

((1->2)-beta-D-glucosyl)(n) + H(2)O = sophorose + ((1->2)-beta-D-glucosyl)(n-2)

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.

A 3'-ribonucleotide + H(2)O = a ribonucleoside + phosphate

O-phospho-L(or D)-serine + H(2)O = L(or D)-serine + phosphate

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine

CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]

ATP + protoporphyrin IX + Mg(2+) + H(2)O = ADP + phosphate + Mg-protoporphyrin IX + 2 H(+)

S-formylglutathione + H(2)O = glutathione + formate

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate

2 L-tyrosyl-tRNA(Tyr) = 2 tRNA(Tyr) + cyclo(L-tyrosyl-L-tyrosyl)

L-lysine + 2-oxoglutarate = (S)-2-amino-6-oxohexanoate + L-glutamate

Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2Z,6E)-farnesyl diphosphate

2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

ATP + [biotin carboxyl-carrier protein]-biotin-N(6)-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N(6)-L-lysine

Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.

A primary alcohol + 2 cytochrome c(L) = an aldehyde + 2 reduced cytochrome c(L) + 2 H(+)

ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol hexakisphosphate

RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O

NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]

ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA

L-arginine + H(2)O = L-ornithine + urea

ATP-dependent cleavage of peptide bonds with broad specificity.

ATP + benzoate + CoA = AMP + diphosphate + benzoyl-CoA

A 2'-deoxyribonucleoside 5'-monophosphate + H(2)O = a 2'-deoxyribonucleoside + phosphate

Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose

Endonucleolytic cleavage to 5'-phosphomonoester.

Androstan-3-alpha,17-beta-diol + NAD(+) = 17-beta-hydroxyandrostan-3-one + NADH

1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate

dTDP-beta-L-rhamnose + NADP(+) = dTDP-4-dehydro-beta-L-rhamnose + NADPH

(1a) NAD(+) = cyclic ADP-ribose + nicotinamide

5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine

(1a) NADP(+) = 2'-phospho-cyclic ADP-ribose + nicotinamide

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)

Tuberculosinyl diphosphate + adenosine = 1-tuberculosinyladenosine + diphosphate

NAD(+) + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H(2)O

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine

Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]

Adenosylcobalamin 5'-phosphate + H(2)O = coenzyme B12 + phosphate

ATP + RNA(n) = diphosphate + RNA(n+1)

Angiotensin II + H(2)O = angiotensin-(1-7) + L-phenylalanine

2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends

S-adenosyl-L-methionine + guanosine(2251) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine(2251) in 23S rRNA 

NAD(+) + H(2)O = ADP-D-ribose + nicotinamide

Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate 

Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + NAD(+) = 3-alpha,12-alpha-dihydroxy-7-oxo-5-beta-cholanate + NADH

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose

Hydrolysis of terminal non-reducing alpha-L-rhamnose residues in alpha-L-rhamnosides

[Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2)

(1a) NAD(+) + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + beta-nicotinamide D-nucleotide

ATP + 3-methylbut-3-en-1-yl phosphate = ADP + 3-methylbut-3-en-1-yl diphosphate

Cutin + H(2)O = cutin monomers

(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate + H(2)O = 3-((3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl)propanoate + (2Z,4Z)-2-hydroxyhexa-2,4-dienoate

D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light

GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + NADPH

2,6-dioxo-6-phenylhexa-3-enoate + H(2)O = benzoate + 2-oxopent-4-enoate

[Amino group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + H(2)O = [amino group carrier protein]-C-terminal-L-glutamate + L-lysine

Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.

D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH

NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine

(1a) (2R,3S)-3-isopropylmalate = 2-isopropylmaleate + H(2)O

Mycoredoxin + ROOH = mycoredoxin disulfide + H(2)O + ROH

[F-actin]-L-methionine + NADPH + O(2) + H(+) = [F-actin]-L-methionine-(R)-S-oxide + NADP(+) + H(2)O

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides

Hg + NADP(+) + H(+) = Hg(2+) + NADPH

L-histidine-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-((3S)-3-amino-3-carboxypropyl)-L-histidine-[translation elongation factor 2] + S-methyl-5'-thioadenosine

10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide

An aldehyde + H(2)O + 2 oxidized ferredoxin = a carboxylate + 2 H(+) + 2 reduced ferredoxin

Preferential cleavage: Glu-|-, Asp-|-.

IMP + H(2)O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide

4a-hydroxytetrahydrobiopterin = 6,7-dihydrobiopterin + H(2)O

(1a) (2R,3S)-3-isopropylmalate + NAD(+) = (2S)-2-isopropyl-3-oxosuccinate + NADH

NAD(P)H + a quinone = NAD(P)(+) + a hydroquinone

Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.

3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O(2) = 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide

A primary alcohol + NAD(+) = an aldehyde + NADH

(1a) 2 Fe(2+) + O(2) + 4 H(2)O = 2 (FeO(OH)) + 4 H(+) + H(2)O(2)

D-glyceraldehyde 3-phosphate = glycerone phosphate

A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + a UDP-3-O-((3R)-hydroxyacyl)-alpha-D-glucosamine = a UDP-2-N,3-O-bis((3R)-3-hydroxyacyl)-alpha-D-glucosamine + a holo-[acyl-carrier-protein]

Naphthalene + NADH + O(2) = (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD(+)

N-succinyl-L-2,6-diaminoheptanedioate + 2-oxoglutarate = N-succinyl-2-L-amino-6-oxoheptanedioate + L-glutamate

ATP + L-aspartate = ADP + 4-phospho-L-aspartate

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline

2 ferrocytochrome c + H(2)O(2) = 2 ferricytochrome c + 2 H(2)O

ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA

(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH

Acetaldehyde + CoA + NAD(+) = acetyl-CoA + NADH

An acylphosphate + H(2)O = a carboxylate + phosphate

Propanal + CoA + NAD(+) = propanoyl-CoA + NADH

ADP + ATP = phosphate + P(1),P(4)-bis(5'-adenosyl) tetraphosphate

NADH + ubiquinone + 5 H(+)(Side 1) = NAD(+) + ubiquinol + 4 H(+)(Side 2)

Acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein]

(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(27) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(27)

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.

Cinnamaldehyde + CoA + NADP(+) = cinnamoyl-CoA + NADPH

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.

Arsenate + glutathione + glutaredoxin = arsenite + a glutaredoxin-glutathione disulfide + H(2)O

Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H(2)O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate

ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein]

Urea + H(2)O = CO(2) + 2 NH(3)

Reduced riboflavin + NAD(P)(+) = riboflavin + NAD(P)H

UDP-N-acetyl-alpha-D-glucosamine = UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H(2)O

NH(3) + 2 H(2)O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H(+)

A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate

D-ribose 5-phosphate = D-ribulose 5-phosphate

S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA 

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol

Thiosulfate + cyanide = sulfite + thiocyanate

N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = N-acetyl-L-glutamyl 5-phosphate + NADPH

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH

Catechol + O(2) = cis,cis-muconate

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate

L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H