4v7e

Electron Microscopy
5.5Å resolution

Model of the small subunit RNA based on a 5.5 A cryo-EM map of Triticum aestivum translating 80S ribosome

Released:

Function and Biology Details

Reactions catalysed:
Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate
(S)-ureidoglycolate = glyoxylate + urea
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Hydrogen sulfide + 6 oxidized ferredoxin [iron-sulfur] cluster + 3 H(2)O = sulfite + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H(+)
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
(1a) cholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = (25S)-26-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO(2)
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
ATP + adenosine = ADP + AMP
ATP + thymidine = ADP + thymidine 5'-phosphate
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate
ATP + thiamine = AMP + thiamine diphosphate
ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate
Creatinine + H(2)O = creatine
5-hydroxyisourate + H(2)O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
Palmitoyl-CoA + H(2)O = CoA + palmitate
CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-
Prephenate = phenylpyruvate + H(2)O + CO(2)
NTP + H(2)O = NDP + phosphate
(Polyphosphate)(n) + H(2)O = (polyphosphate)(n-1) + phosphate
1-haloalkane + H(2)O = a primary alcohol + halide
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate
ATP + deamido-NAD(+) + L-glutamine + H(2)O = AMP + diphosphate + NAD(+) + L-glutamate
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate
Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
RX + glutathione = HX + R-S-glutathione
6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate
ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide
Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
Acetyl-CoA + H(2)O + glyoxylate = (S)-malate + CoA
Arsenate + mycothiol = arseno-mycothiol + H(2)O
Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O
4-fumarylacetoacetate + H(2)O = acetoacetate + fumarate
Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO(2) 
(1a) L-serine = 2-aminoprop-2-enoate + H(2)O
dUTP + H(2)O = dUMP + diphosphate
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate
ATP + a protein = ADP + a phosphoprotein
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Chorismate = prephenate
NDP-glucose + 3-phospho-D-glycerate = NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
[Biotin carboxyl-carrier protein]-N(6)-carboxybiotinyl-L-lysine + acetyl-CoA = [biotin carboxyl-carrier protein]-N(6)-biotinyl-L-lysine + malonyl-CoA
Aceneneuramate = N-acetyl-D-mannosamine + pyruvate
A beta-lactam + H(2)O = a substituted beta-amino acid
D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H(2)O
2 bilirubin + O(2) = 2 biliverdin + 2 H(2)O
ATP + UMP = ADP + UDP
L-aspartate 4-semialdehyde + phosphate + NADP(+) = L-4-aspartyl phosphate + NADPH
L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and similar substrates, releasing cellobiose from the reducing ends of the chains.
NAD(P)H + O(2) = NAD(P)(+) + H(2)O(2)
Succinate + a quinone = fumarate + a quinol
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH = [CysO sulfur-carrier protein]-Gly-NH-CH(2)-C(O)-S-L-cysteine + phosphate
Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
ATP + H(2)O = ADP + phosphate
Release of N-terminal proline from a peptide.
10-formyltetrahydrofolate + H(2)O = formate + tetrahydrofolate
GTP + H(2)O = GDP + phosphate
Cleavage of peptide bonds with very broad specificity.
2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate
A phenyl acetate + H(2)O = a phenol + acetate
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
A [lipoyl-carrier protein]-N(6)-((R)-dihydrolipoyl)-L-lysine + ROOH = a [lipoyl-carrier protein]-N(6)-((R)-lipoyl)-L-lysine + H(2)O + ROH
An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).
Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate
4-(2-carboxyphenyl)-4-oxobutanoyl-CoA = 1,4-dihydroxy-2-naphthoyl-CoA + H(2)O
Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
NADPH + NAD(+) + H(+)(Side 1) = NADP(+) + H(+)(Side 2) + NADH
N-carbamoylputrescine + H(2)O = putrescine + CO(2) + NH(3)
2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
(+)-muconolactone = 5-oxo-4,5-dihydrofuran-2-acetate
5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+)
ATP + dTMP = ADP + dTDP
L-alanine + H(2)O + NAD(+) = pyruvate + NH(3) + NADH
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate
Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose
5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H(2)O
ATP + 7,8-diaminononanoate + CO(2) = ADP + phosphate + dethiobiotin
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate = cis,cis-butadiene-1,2,4-tricarboxylate
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
S-adenosyl-L-homocysteine + H(2)O = L-homocysteine + adenosine
Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate
Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
AMP + H(2)O = D-ribose 5-phosphate + adenine
7,8-dihydroneopterin 3'-triphosphate + H(2)O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
N-acetyl-O-acetylneuraminate + H(2)O = N-acetylneuraminate + acetate
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine
(S)-lactate + NAD(+) = pyruvate + NADH
Diphosphate + H(2)O = 2 phosphate
L-lysine + NADPH + O(2) = N(6)-hydroxy-L-lysine + NADP(+) + H(2)O
Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH
(S)-dihydroorotate + fumarate = orotate + succinate
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
3-deoxy-D-manno-octulosonate 8-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate + phosphate
2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH
ATP = 3',5'-cyclic AMP + diphosphate
Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO(2)
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2)
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H(2)O = tetrahydrofolate + 2-dehydropantoate
L-alanine = D-alanine
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Pyridoxamine 5'-phosphate + H(2)O + O(2) = pyridoxal 5'-phosphate + NH(3) + H(2)O(2)
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH(2) + O(2) = 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H(2)O
3-phospho-D-glycerate + NAD(+) = 3-phosphonooxypyruvate + NADH
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine
(1a) [enzyme]-L-histidine + 2,3-bisphospho-D-glycerate = [enzyme]-N(tau)-phospho-L-histidine + 2/3-phospho-D-glycerate
2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + NADPH
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + H(2)O = 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + acetate
Release of an N-terminal D-amino acid from a peptide, Xaa-|-Yaa-, in which Xaa is preferably D-Ala, D-Ser or D-Thr. D-amino acid amides and methyl esters also are hydrolyzed, as is glycine amide.
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
2-oxo-2H-pyran-4,6-dicarboxylate + H(2)O = (1E)-4-oxobut-1-ene-1,2,4-tricarboxylate
Phosphonoacetaldehyde + H(2)O = acetaldehyde + phosphate
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Hydrolysis of the bonds linking certain hydrophobic residues in hemoglobin or globin. Also cleaves small molecules substrates such as Ala-Leu-Glu-Arg-Thr-Phe-|-Phe(NO(2))-Ser-Phe-Pro-Thr.
Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites
Isochorismate + H(2)O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
D-arabinose 5-phosphate = D-ribulose 5-phosphate
(R)-2-hydroxyglutarate + NAD(+) = 2-oxoglutarate + NADH
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
2 H(2)O(2) = O(2) + 2 H(2)O
(S)-malate = fumarate + H(2)O
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
ATP + D-ribose = ADP + D-ribose 5-phosphate
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
ATP + L-phenylalanine + H(2)O = AMP + diphosphate + D-phenylalanine
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
An ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO(2) + a holo-[acyl-carrier protein]
L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH
Adenosine + H(2)O = inosine + NH(3)
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate
Alpha-D-glucose = beta-D-glucose
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO(2) + H(2)O
Beta-nicotinate D-ribonucleotide + diphosphate + CO(2) = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate
2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
N(6)-(Delta(2)-isopentenyl)-adenosine 5'-phosphate + H(2)O = N(6)-(dimethylallyl)adenine + D-ribose 5'-phosphate
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
Beta-D-ribopyranose = beta-D-ribofuranose
ATP + glycerol = ADP + sn-glycerol 3-phosphate
(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine
L-lysine = cadaverine + CO(2)
UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
ATP + shikimate = ADP + shikimate 3-phosphate
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Peptidylproline (omega=180) = peptidylproline (omega=0)
Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol
Protein L-glutamine + H(2)O = protein L-glutamate + NH(3)
Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
ATP + (R)-pantothenate = ADP + (R)-4'-phosphopantothenate
NADH + ROOH + H(+) = NAD(+) + H(2)O + ROH
A 5'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
H(2)CO(3) = CO(2) + H(2)O
Acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N(3)-acetyl-2-deoxystreptamine antibiotic
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides
4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate
Myo-inositol phosphate + H(2)O = myo-inositol + phosphate
L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate + NH(3) + NADPH
Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans
A phosphatidylcholine + H(2)O = 1,2-diacyl-sn-glycerol + phosphocholine
(3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H(2)O
A chalcone = a flavanone
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Protoheme + 3 reduced acceptor + 3 O(2) = mycobilin a + Fe(2+) + 3 acceptor + 3 H(2)O
ATP + H(2)O + H(+)(Side 1) + K(+)(Side 2) = ADP + phosphate + H(+)(Side 2) + K(+)(Side 1)
Succinyl-CoA + (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + H(2)O = CoA + N-succinyl-L-2-amino-6-oxoheptanedioate
2-iminobutanoate + H(2)O = 2-oxobutanoate + NH(3)
Succinyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-succinyldihydrolipoyl)lysine
Cyclo(L-tyrosyl-L-tyrosyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = mycocyclosin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H(2)O
Isocitrate = succinate + glyoxylate
2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
Quercetin + O(2) = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H(+)
Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine
Choline = trimethylamine + acetaldehyde
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
D-galactose + O(2) = D-galacto-hexodialdose + H(2)O(2)
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH
(R)-10-hydroxystearate = oleate + H(2)O
(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate
An aldehyde + NAD(P)(+) + H(2)O = a carboxylate + NAD(P)H
N(6)-prenyladenine + acceptor + H(2)O = adenine + 3-methylbut-2-enal + reduced acceptor
(2S)-2-hydroxy-3,4-dioxopentyl phosphate = 3-hydroxy-2,4-dioxopentyl phosphate
Hydrolyzes poly(ADP-D-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-D-ribose
(1a) 2 cob(II)alamin + 2 [corrinoid adenosyltransferase] = 2 [corrinoid adenosyltransferase]-cob(II)alamin
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + ditrans,polycis-polyprenyl diphosphate (n = 10-55)
Chorismate = isochorismate
L-arginine + 2-oxoglutarate + O(2) = (3S)-3-hydroxy-L-arginine + succinate + CO(2)
ATP + phenylacetate + CoA = AMP + diphosphate + phenylacetyl-CoA
3-oxoadipate + NAD(P)(+) = 2-maleylacetate + NAD(P)H
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Isochorismate = salicylate + pyruvate
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
2 glutathione + ROOH = glutathione disulfide + H(2)O + ROH
Maltose = alpha,alpha-trehalose
Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.
5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H
6-phospho-D-glucono-1,5-lactone + H(2)O = 6-phospho-D-gluconate
D-xylopyranose = D-xylulose
ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate
2 nitric oxide + 2 O(2) + NAD(P)H = 2 nitrate + NAD(P)(+) + H(+)
3-dehydroquinate = 3-dehydroshikimate + H(2)O
S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)(+) + H(2)O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H
S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA 
Nucleoside 2',3'-cyclic phosphate + H(2)O = nucleoside 3'-phosphate
Nucleoside 3',5'-cyclic phosphate + H(2)O = nucleoside 5'-phosphate
S-adenosyl-L-methionine + pseudouridine(1915) in 23S rRNA = S-adenosyl-L-homocysteine + N(3)-methylpseudouridine(1915) in 23S rRNA
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate
4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO(2)
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
Succinate semialdehyde + NAD(P)(+) + H(2)O = succinate + NAD(P)H
1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
ATP + pyruvate = ADP + phosphoenolpyruvate
ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
GTP = 3',5'-cyclic GMP + diphosphate
ADP-alpha-D-glucose + D-glucose 6-phosphate = ADP + alpha,alpha-trehalose 6-phosphate
Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-|-Xaa- and -Yaa-Xaa-Gly-Xaa-|-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid).
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
An ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO(2) + a holo-[acyl-carrier protein]
ATP + AMP = 2 ADP
Trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD = trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH(2)
L-histidinol phosphate + H(2)O = L-histidinol + phosphate
Pectin + n H(2)O = n methanol + pectate
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Blasticidin S + H(2)O = deaminohydroxyblasticidin S + NH(3)
ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA
The primary cleavage site is at 67-His-|-Lys-68 in human C5a with a minor secondary cleavage site at 58-Ala-|-Ser-59
A phosphate monoester + H(2)O = an alcohol + phosphate
dCTP + 2 H(2)O = dUMP + diphosphate + NH(3)
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H(2)O = 1-alkyl-sn-glycero-3-phosphocholine + acetate
D-glucose 6-phosphate + oxidized coenzyme F420 = D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420
An alpha-L-fucoside + H(2)O = L-fucose + an alcohol
(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O
L-lysyl-tRNA(Lys) + phosphatidylglycerol = tRNA(Lys) + 3-O-L-lysyl-1-O-phosphatidylglycerol
GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose
ATP + NAD(+) = ADP + NADP(+)
L-asparagine + H(2)O = L-aspartate + NH(3)
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
2-phosphoglycolate + H(2)O = glycolate + phosphate
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Acetyl-CoA + phosphate = CoA + acetyl phosphate
Acetyl-CoA + 3-methyl-2-oxobutanoate + H(2)O = (2S)-2-isopropylmalate + CoA
ATP + pyridoxal = ADP + pyridoxal 5'-phosphate
((1->2)-beta-D-glucosyl)(n) + H(2)O = sophorose + ((1->2)-beta-D-glucosyl)(n-2)
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
A 3'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
O-phospho-L(or D)-serine + H(2)O = L(or D)-serine + phosphate
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]
ATP + protoporphyrin IX + Mg(2+) + H(2)O = ADP + phosphate + Mg-protoporphyrin IX + 2 H(+)
S-formylglutathione + H(2)O = glutathione + formate
Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
2 L-tyrosyl-tRNA(Tyr) = 2 tRNA(Tyr) + cyclo(L-tyrosyl-L-tyrosyl)
L-lysine + 2-oxoglutarate = (S)-2-amino-6-oxohexanoate + L-glutamate
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2Z,6E)-farnesyl diphosphate
2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
ATP + [biotin carboxyl-carrier protein]-biotin-N(6)-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N(6)-L-lysine
Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.
A primary alcohol + 2 cytochrome c(L) = an aldehyde + 2 reduced cytochrome c(L) + 2 H(+)
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol hexakisphosphate
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA
L-arginine + H(2)O = L-ornithine + urea
ATP-dependent cleavage of peptide bonds with broad specificity.
ATP + benzoate + CoA = AMP + diphosphate + benzoyl-CoA
A 2'-deoxyribonucleoside 5'-monophosphate + H(2)O = a 2'-deoxyribonucleoside + phosphate
Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Endonucleolytic cleavage to 5'-phosphomonoester.
Androstan-3-alpha,17-beta-diol + NAD(+) = 17-beta-hydroxyandrostan-3-one + NADH
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate
dTDP-beta-L-rhamnose + NADP(+) = dTDP-4-dehydro-beta-L-rhamnose + NADPH
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
(1a) NADP(+) = 2'-phospho-cyclic ADP-ribose + nicotinamide
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Tuberculosinyl diphosphate + adenosine = 1-tuberculosinyladenosine + diphosphate
NAD(+) + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H(2)O
S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
Adenosylcobalamin 5'-phosphate + H(2)O = coenzyme B12 + phosphate
ATP + RNA(n) = diphosphate + RNA(n+1)
Angiotensin II + H(2)O = angiotensin-(1-7) + L-phenylalanine
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends
S-adenosyl-L-methionine + guanosine(2251) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine(2251) in 23S rRNA 
NAD(+) + H(2)O = ADP-D-ribose + nicotinamide
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate 
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + NAD(+) = 3-alpha,12-alpha-dihydroxy-7-oxo-5-beta-cholanate + NADH
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose
Hydrolysis of terminal non-reducing alpha-L-rhamnose residues in alpha-L-rhamnosides
[Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2)
(1a) NAD(+) + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + beta-nicotinamide D-nucleotide
ATP + 3-methylbut-3-en-1-yl phosphate = ADP + 3-methylbut-3-en-1-yl diphosphate
Cutin + H(2)O = cutin monomers
(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate + H(2)O = 3-((3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl)propanoate + (2Z,4Z)-2-hydroxyhexa-2,4-dienoate
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + NADPH
2,6-dioxo-6-phenylhexa-3-enoate + H(2)O = benzoate + 2-oxopent-4-enoate
[Amino group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + H(2)O = [amino group carrier protein]-C-terminal-L-glutamate + L-lysine
Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine
(1a) (2R,3S)-3-isopropylmalate = 2-isopropylmaleate + H(2)O
Mycoredoxin + ROOH = mycoredoxin disulfide + H(2)O + ROH
[F-actin]-L-methionine + NADPH + O(2) + H(+) = [F-actin]-L-methionine-(R)-S-oxide + NADP(+) + H(2)O
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Hg + NADP(+) + H(+) = Hg(2+) + NADPH
L-histidine-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-((3S)-3-amino-3-carboxypropyl)-L-histidine-[translation elongation factor 2] + S-methyl-5'-thioadenosine
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide
An aldehyde + H(2)O + 2 oxidized ferredoxin = a carboxylate + 2 H(+) + 2 reduced ferredoxin
Preferential cleavage: Glu-|-, Asp-|-.
IMP + H(2)O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
4a-hydroxytetrahydrobiopterin = 6,7-dihydrobiopterin + H(2)O
(1a) (2R,3S)-3-isopropylmalate + NAD(+) = (2S)-2-isopropyl-3-oxosuccinate + NADH
NAD(P)H + a quinone = NAD(P)(+) + a hydroquinone
Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O(2) = 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
A primary alcohol + NAD(+) = an aldehyde + NADH
(1a) 2 Fe(2+) + O(2) + 4 H(2)O = 2 (FeO(OH)) + 4 H(+) + H(2)O(2)
D-glyceraldehyde 3-phosphate = glycerone phosphate
A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + a UDP-3-O-((3R)-hydroxyacyl)-alpha-D-glucosamine = a UDP-2-N,3-O-bis((3R)-3-hydroxyacyl)-alpha-D-glucosamine + a holo-[acyl-carrier-protein]
Naphthalene + NADH + O(2) = (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD(+)
N-succinyl-L-2,6-diaminoheptanedioate + 2-oxoglutarate = N-succinyl-2-L-amino-6-oxoheptanedioate + L-glutamate
ATP + L-aspartate = ADP + 4-phospho-L-aspartate
Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
2 ferrocytochrome c + H(2)O(2) = 2 ferricytochrome c + 2 H(2)O
ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Acetaldehyde + CoA + NAD(+) = acetyl-CoA + NADH
An acylphosphate + H(2)O = a carboxylate + phosphate
Propanal + CoA + NAD(+) = propanoyl-CoA + NADH
ADP + ATP = phosphate + P(1),P(4)-bis(5'-adenosyl) tetraphosphate
NADH + ubiquinone + 5 H(+)(Side 1) = NAD(+) + ubiquinol + 4 H(+)(Side 2)
Acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein]
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(27) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(27)
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
Cinnamaldehyde + CoA + NADP(+) = cinnamoyl-CoA + NADPH
Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.
Arsenate + glutathione + glutaredoxin = arsenite + a glutaredoxin-glutathione disulfide + H(2)O
Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H(2)O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein]
Urea + H(2)O = CO(2) + 2 NH(3)
Reduced riboflavin + NAD(P)(+) = riboflavin + NAD(P)H
UDP-N-acetyl-alpha-D-glucosamine = UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H(2)O
NH(3) + 2 H(2)O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H(+)
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
D-ribose 5-phosphate = D-ribulose 5-phosphate
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA 
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
Thiosulfate + cyanide = sulfite + thiocyanate
N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = N-acetyl-L-glutamyl 5-phosphate + NADPH
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH
Catechol + O(2) = cis,cis-muconate
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 88-mer (preferred)
Entry contents:
80 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (86 distinct):
40S ribosomal protein S24E Chain: BY
Molecule details ›
Chain: BY
Length: 138 amino acids
Theoretical weight: 15.79 KDa
Source organism: Triticum aestivum
40S ribosomal protein S8E Chain: BI
Molecule details ›
Chain: BI
Length: 220 amino acids
Theoretical weight: 24.88 KDa
Source organism: Triticum aestivum
40S ribosomal protein S10E Chain: BK
Molecule details ›
Chain: BK
Length: 183 amino acids
Theoretical weight: 20.29 KDa
Source organism: Triticum aestivum
40S ribosomal protein S12E Chain: BM
Molecule details ›
Chain: BM
Length: 171 amino acids
Theoretical weight: 18.4 KDa
Source organism: Triticum aestivum
40S ribosomal protein S31e Chain: Bf
Molecule details ›
Chain: Bf
Length: 155 amino acids
Theoretical weight: 17.72 KDa
Source organism: Triticum aestivum
40S ribosomal protein S12 Chain: BX
Molecule details ›
Chain: BX
Length: 142 amino acids
Theoretical weight: 15.7 KDa
Source organism: Triticum aestivum
G protein beta subunit Chain: Bg
Molecule details ›
Chain: Bg
Length: 380 amino acids
Theoretical weight: 41.79 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q8LNY6 (Residues: 1-380; Coverage: 100%)
Gene name: TaGB1
Sequence domains: WD domain, G-beta repeat
KH type-2 domain-containing protein Chain: BD
Molecule details ›
Chain: BD
Length: 208 amino acids
Theoretical weight: 23.26 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q8L804 (Residues: 12-219; Coverage: 92%)
Sequence domains:
40S ribosomal protein S4E Chain: BE
Molecule details ›
Chain: BE
Length: 265 amino acids
Theoretical weight: 29.92 KDa
Source organism: Triticum aestivum
40S ribosomal protein S7 Chain: BF
Molecule details ›
Chain: BF
Length: 191 amino acids
Theoretical weight: 21.25 KDa
Source organism: Triticum aestivum
40S ribosomal protein S9 Chain: BQ
Molecule details ›
Chain: BQ
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Triticum aestivum
40S ribosomal protein S10 Chain: BU
Molecule details ›
Chain: BU
Length: 128 amino acids
Theoretical weight: 14.07 KDa
Source organism: Triticum aestivum
40S ribosomal protein S11 Chain: BO
Molecule details ›
Chain: BO
Length: 151 amino acids
Theoretical weight: 16.35 KDa
Source organism: Triticum aestivum
Putative ribosomal protein S18 Chain: BS
Molecule details ›
Chain: BS
Length: 152 amino acids
Theoretical weight: 17.7 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q8L806 (Residues: 1-152; Coverage: 100%)
Sequence domains: Ribosomal protein S13/S18
40S ribosomal protein S15 Chain: BN
Molecule details ›
Chain: BN
Length: 151 amino acids
Theoretical weight: 17.15 KDa
Source organism: Triticum aestivum
40S ribosomal protein S17 Chain: BL
Molecule details ›
Chain: BL
Length: 160 amino acids
Theoretical weight: 18.03 KDa
Source organism: Triticum aestivum
40S ribosomal protein S19E Chain: BT
Molecule details ›
Chain: BT
Length: 146 amino acids
Theoretical weight: 16.41 KDa
Source organism: Triticum aestivum
40S ribosomal protein S19 Chain: BP
Molecule details ›
Chain: BP
Length: 154 amino acids
Theoretical weight: 17.45 KDa
Source organism: Triticum aestivum
40S ribosomal protein S25E Chain: BZ
Molecule details ›
Chain: BZ
Length: 108 amino acids
Theoretical weight: 11.94 KDa
Source organism: Triticum aestivum
S28 ribosomal protein Chain: Bc
Molecule details ›
Chain: Bc
Length: 65 amino acids
Theoretical weight: 7.48 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q7X9K4 (Residues: 22-86; Coverage: 76%)
Sequence domains: Ribosomal protein S28e
40S ribosomal protein S8 Chain: BW
Molecule details ›
Chain: BW
Length: 130 amino acids
Theoretical weight: 14.83 KDa
Source organism: Triticum aestivum
40S ribosomal protein S29 Chain: Bd
Molecule details ›
Chain: Bd
Length: 56 amino acids
Theoretical weight: 6.39 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7K3 (Residues: 1-56; Coverage: 100%)
Gene name: RPS29
Sequence domains: Ribosomal protein S14p/S29e
40S ribosomal protein S27E Chain: Bb
Molecule details ›
Chain: Bb
Length: 86 amino acids
Theoretical weight: 9.54 KDa
Source organism: Triticum aestivum
40S ribosomal protein S30E Chain: Be
Molecule details ›
Chain: Be
Length: 62 amino acids
Theoretical weight: 6.95 KDa
Source organism: Triticum aestivum
40S ribosomal protein S2 Chain: BA
Molecule details ›
Chain: BA
Length: 260 amino acids
Theoretical weight: 28.72 KDa
Source organism: Triticum aestivum
40S ribosomal protein S17E Chain: BR
Molecule details ›
Chain: BR
Length: 141 amino acids
Theoretical weight: 16.46 KDa
Source organism: Triticum aestivum
40S ribosomal protein S1E Chain: BB
Molecule details ›
Chain: BB
Length: 262 amino acids
Theoretical weight: 29.78 KDa
Source organism: Triticum aestivum
40S ribosomal protein S21E Chain: BV
Molecule details ›
Chain: BV
Length: 82 amino acids
Theoretical weight: 9.27 KDa
Source organism: Triticum aestivum
40S ribosomal protein S26E Chain: Ba
Molecule details ›
Chain: Ba
Length: 133 amino acids
Theoretical weight: 15.03 KDa
Source organism: Triticum aestivum
40S ribosomal protein S4 Chain: BJ
Molecule details ›
Chain: BJ
Length: 195 amino acids
Theoretical weight: 22.63 KDa
Source organism: Triticum aestivum
40S ribosomal protein S5 Chain: BC
Molecule details ›
Chain: BC
Length: 263 amino acids
Theoretical weight: 28.86 KDa
Source organism: Triticum aestivum
40S ribosomal protein S6 Chain: BG
Molecule details ›
Chain: BG
Length: 245 amino acids
Theoretical weight: 28.11 KDa
Source organism: Triticum aestivum
40S ribosomal protein S7 Chain: BH
Molecule details ›
Chain: BH
Length: 189 amino acids
Theoretical weight: 21.9 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7K2 (Residues: 4-192; Coverage: 98%)
Sequence domains: Ribosomal protein S7e
60S ribosomal protein L8E Chain: CG
Molecule details ›
Chain: CG
Length: 257 amino acids
Theoretical weight: 29.24 KDa
Source organism: Triticum aestivum
60S ribosomal protein L21 Chain: CT
Molecule details ›
Chain: CT
Length: 164 amino acids
Theoretical weight: 18.72 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: F4YWL9 (Residues: 1-164; Coverage: 100%)
Gene name: RPL21
Sequence domains: Ribosomal protein L21e
60S ribosomal protein L27E Chain: CZ
Molecule details ›
Chain: CZ
Length: 136 amino acids
Theoretical weight: 15.51 KDa
Source organism: Triticum aestivum
Ribosomal protein Chain: Cz
Molecule details ›
Chain: Cz
Length: 216 amino acids
Theoretical weight: 24.6 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7L3 (Residues: 1-216; Coverage: 100%)
Sequence domains: Ribosomal protein L1p/L10e family
60S ribosomal protein L2 Chain: CA
Molecule details ›
Chain: CA
Length: 261 amino acids
Theoretical weight: 28.31 KDa
Source organism: Triticum aestivum
Ribosomal protein L11 Chain: CJ
Molecule details ›
Chain: CJ
Length: 180 amino acids
Theoretical weight: 20.73 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7L2 (Residues: 1-180; Coverage: 100%)
Sequence domains:
60S ribosomal protein L6 Chain: CH
Molecule details ›
Chain: CH
Length: 190 amino acids
Theoretical weight: 21.38 KDa
Source organism: Triticum aestivum
Ribosomal protein L17 Chain: CV
Molecule details ›
Chain: CV
Length: 140 amino acids
Theoretical weight: 15.04 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7K4 (Residues: 1-140; Coverage: 100%)
Sequence domains: Ribosomal protein L14p/L23e
60S ribosomal protein L15E Chain: CN
Molecule details ›
Chain: CN
Length: 200 amino acids
Theoretical weight: 23.76 KDa
Source organism: Triticum aestivum
60S ribosomal protein L15 Chain: Ca
Molecule details ›
Chain: Ca
Length: 144 amino acids
Theoretical weight: 15.83 KDa
Source organism: Triticum aestivum
Ribosomal_L18e/L15P domain-containing protein Chain: CQ
Molecule details ›
Chain: CQ
Length: 188 amino acids
Theoretical weight: 21.38 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7L0 (Residues: 1-188; Coverage: 100%)
Sequence domains: Ribosomal protein 60S L18 and 50S L18e
60S ribosomal protein L18 Chain: CD
Molecule details ›
Chain: CD
Length: 304 amino acids
Theoretical weight: 34.72 KDa
Source organism: Triticum aestivum
Ribosomal protein L19 Chain: CR
Molecule details ›
Chain: CR
Length: 209 amino acids
Theoretical weight: 24.49 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q7XY20 (Residues: 1-209; Coverage: 100%)
Sequence domains: Ribosomal protein L19e
60S ribosomal protein L22 Chain: CP
Molecule details ›
Chain: CP
Length: 171 amino acids
Theoretical weight: 19.54 KDa
Source organism: Triticum aestivum
60S ribosomal protein L23 Chain: CX
Molecule details ›
Chain: CX
Length: 152 amino acids
Theoretical weight: 17.09 KDa
Source organism: Triticum aestivum
60S ribosomal protein L24E Chain: CW
Molecule details ›
Chain: CW
Length: 162 amino acids
Theoretical weight: 18.5 KDa
Source organism: Triticum aestivum
60S ribosomal protein L24 Chain: CY
Molecule details ›
Chain: CY
Length: 150 amino acids
Theoretical weight: 16.97 KDa
Source organism: Triticum aestivum
60S ribosomal protein L28E Chain: Cr
Molecule details ›
Chain: Cr
Length: 147 amino acids
Theoretical weight: 16.64 KDa
Source organism: Triticum aestivum
60S ribosomal protein L30 Chain: Cc
Molecule details ›
Chain: Cc
Length: 112 amino acids
Theoretical weight: 12.29 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7K9 (Residues: 1-112; Coverage: 100%)
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
60S ribosomal protein L31E Chain: Cd
Molecule details ›
Chain: Cd
Length: 123 amino acids
Theoretical weight: 14.09 KDa
Source organism: Triticum aestivum
60S ribosomal protein L32E Chain: Ce
Molecule details ›
Chain: Ce
Length: 133 amino acids
Theoretical weight: 15.74 KDa
Source organism: Triticum aestivum
60S ribosomal protein L37E Chain: Cj
Molecule details ›
Chain: Cj
Length: 94 amino acids
Theoretical weight: 10.82 KDa
Source organism: Triticum aestivum
Ribosomal protein L39 Chain: Cl
Molecule details ›
Chain: Cl
Length: 51 amino acids
Theoretical weight: 6.53 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7K7 (Residues: 1-51; Coverage: 100%)
Sequence domains: Ribosomal L39 protein
60S ribosomal protein L44E Chain: Co
Molecule details ›
Chain: Co
Length: 105 amino acids
Theoretical weight: 12.17 KDa
Source organism: Triticum aestivum
60S ribosomal protein L14E Chain: CM
Molecule details ›
Chain: CM
Length: 134 amino acids
Theoretical weight: 15.42 KDa
Source organism: Triticum aestivum
60S ribosomal protein L20 Chain: CS
Molecule details ›
Chain: CS
Length: 178 amino acids
Theoretical weight: 21.44 KDa
Source organism: Triticum aestivum
60S ribosomal protein L22E Chain: CU
Molecule details ›
Chain: CU
Length: 130 amino acids
Theoretical weight: 14.44 KDa
Source organism: Triticum aestivum
60S ribosomal protein L36 Chain: Ci
Molecule details ›
Chain: Ci
Length: 112 amino acids
Theoretical weight: 12.56 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7L5 (Residues: 1-112; Coverage: 100%)
Sequence domains: Ribosomal protein L36e
60S ribosomal protein L11 Chain: CK
Molecule details ›
Chain: CK
Length: 166 amino acids
Theoretical weight: 17.78 KDa
Source organism: Triticum aestivum
Ribosomal protein P1 Chains: Cu, Cv
Molecule details ›
Chains: Cu, Cv
Length: 110 amino acids
Theoretical weight: 11.2 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7K5 (Residues: 1-110; Coverage: 100%)
Sequence domains: 60s Acidic ribosomal protein
Acidic ribosomal protein P2 Chains: Cs, Ct
Molecule details ›
Chains: Cs, Ct
Length: 113 amino acids
Theoretical weight: 11.6 KDa
Source organism: Triticum aestivum
60S ribosomal protein L35 Chain: Ch
Molecule details ›
Chain: Ch
Length: 124 amino acids
Theoretical weight: 14.4 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q8L805 (Residues: 1-124; Coverage: 100%)
Gene name: RPL35
Sequence domains: Ribosomal L29 protein
Ribosomal protein L7 Chain: CF
Molecule details ›
Chain: CF
Length: 244 amino acids
Theoretical weight: 28.18 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7K6 (Residues: 1-244; Coverage: 100%)
Sequence domains:
60S acidic ribosomal protein P0 Chain: Cq
Molecule details ›
Chain: Cq
Length: 319 amino acids
Theoretical weight: 34.42 KDa
Source organism: Triticum aestivum
Ribosomal protein L3-A1 Chain: CB
Molecule details ›
Chain: CB
Length: 389 amino acids
Theoretical weight: 44.72 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q7X744 (Residues: 1-389; Coverage: 100%)
Gene names: RPL3, RPL3-A1, RPL3A-1
Sequence domains: Ribosomal protein L3
60S ribosomal protein L4 Chain: CC
Molecule details ›
Chain: CC
Length: 405 amino acids
Theoretical weight: 44.82 KDa
Source organism: Triticum aestivum
Ribosomal protein L13a Chain: CO
Molecule details ›
Chain: CO
Length: 206 amino acids
Theoretical weight: 23.58 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7L1 (Residues: 1-206; Coverage: 100%)
Sequence domains: Ribosomal protein L13
60S ribosomal protein L37a, expressed Chain: Cp
Molecule details ›
Chain: Cp
Length: 92 amino acids
Theoretical weight: 10.27 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: D8L9P6 (Residues: 1-92; Coverage: 100%)
Gene names: TAA_ctg0616b.00240.1, TRAES_3BF073800030CFD_c1
Sequence domains: Ribosomal L37ae protein family
60S ribosomal protein L16 Chain: CI
Molecule details ›
Chain: CI
Length: 224 amino acids
Theoretical weight: 25.4 KDa
Source organism: Triticum aestivum
60S ribosomal protein L41E Chain: Cn
Molecule details ›
Chain: Cn
Length: 25 amino acids
Theoretical weight: 3.45 KDa
Source organism: Triticum aestivum
60S ribosomal protein L40E Chain: Cm
Molecule details ›
Chain: Cm
Length: 53 amino acids
Theoretical weight: 6.3 KDa
Source organism: Triticum aestivum
60S ribosomal protein L13E Chain: CL
Molecule details ›
Chain: CL
Length: 208 amino acids
Theoretical weight: 24.07 KDa
Source organism: Triticum aestivum
60S ribosomal protein L6 Chain: CE
Molecule details ›
Chain: CE
Length: 219 amino acids
Theoretical weight: 24.59 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7L4 (Residues: 1-219; Coverage: 100%)
Sequence domains: Ribosomal protein L6e
60S ribosomal protein L33E Chain: Cf
Molecule details ›
Chain: Cf
Length: 111 amino acids
Theoretical weight: 12.68 KDa
Source organism: Triticum aestivum
60S ribosomal protein L38E Chain: Ck
Molecule details ›
Chain: Ck
Length: 69 amino acids
Theoretical weight: 8.05 KDa
Source organism: Triticum aestivum
60S ribosomal protein L29E Chain: Cb
Molecule details ›
Chain: Cb
Length: 60 amino acids
Theoretical weight: 7.07 KDa
Source organism: Triticum aestivum
Ribosomal protein l34 Chain: Cg
Molecule details ›
Chain: Cg
Length: 119 amino acids
Theoretical weight: 13.81 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7K8 (Residues: 1-119; Coverage: 100%)
Sequence domains: Ribosomal protein L34e
18S Ribosomal RNA Chain: Ad
P-site tRNA Chain: Ae
Molecule details ›
Chain: Ae
Length: 75 nucleotides
Theoretical weight: 24.14 KDa
Sequence domains: tRNA
5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' Chain: Af
Molecule details ›
Chain: Af
Length: 11 nucleotides
Theoretical weight: 3.5 KDa
60S ribosomal RNA Chain: Aa
Molecule details ›
Chain: Aa
Length: 3391 nucleotides
Theoretical weight: 1097.29 KDa
Sequence domains:
  • Archaeal large subunit ribosomal RNA
  • Bacterial large subunit ribosomal RNA
  • Eukaryotic large subunit ribosomal RNA
  • 5.8S ribosomal RNA Chain: Ac
    Molecule details ›
    Chain: Ac
    Length: 160 nucleotides
    Theoretical weight: 51.54 KDa
    Sequence domains: 5.8S ribosomal RNA
    5S ribosomal RNA Chain: Ab
    Molecule details ›
    Chain: Ab
    Length: 120 nucleotides
    Theoretical weight: 38.72 KDa
    Sequence domains: 5S ribosomal RNA

    Ligands and Environments

    No bound ligands
    No modified residues

    Experiments and Validation Details

    Entry percentile scores
    Resolution: 5.5Å
    Relevant EMDB volumes: EMD-1780