Structure analysis

Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin and ribosome recycling factor (RRF).

X-ray diffraction
4Å resolution
Assembly composition:
hetero 53-mer (preferred)
Entry contents: 50 distinct polypeptide molecules
3 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 53-mer
Accessible surface area: 757827.72 Å2
Buried surface area: 259926.44 Å2
Dissociation area: 600.12 Å2
Dissociation energy (ΔGdiss): -6.5 kcal/mol
Dissociation entropy (TΔSdiss): 13.67 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-135635
Assembly 2
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Multimeric state: hetero 53-mer
Accessible surface area: 756255.63 Å2
Buried surface area: 263061.04 Å2
Dissociation area: 630.44 Å2
Dissociation energy (ΔGdiss): -3.36 kcal/mol
Dissociation entropy (TΔSdiss): 13.61 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-135635

Macromolecules

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Chains: AH, CH
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W7 (Residues: 2-130; Coverage: 99%)
Gene names: JW3268, b3306, rpsH
Pfam: Ribosomal protein S8
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Chains: AJ, CJ
Length: 103 amino acids
Theoretical weight: 11.76 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R5 (Residues: 1-103; Coverage: 100%)
Gene names: JW3283, b3321, nusE, rpsJ
Pfam: Ribosomal protein S10p/S20e
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Chains: AK, CK
Length: 128 amino acids
Theoretical weight: 13.74 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R9 (Residues: 2-129; Coverage: 99%)
Gene names: JW3259, b3297, rpsK
Pfam: Ribosomal protein S11
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Chains: AL, CL
Length: 123 amino acids
Theoretical weight: 13.64 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 2-124; Coverage: 99%)
Gene names: JW3304, b3342, rpsL, strA
Pfam: Ribosomal protein S12/S23
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Chains: AN, CN
Length: 100 amino acids
Theoretical weight: 11.48 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG59 (Residues: 2-101; Coverage: 99%)
Gene names: JW3269, b3307, rpsN
Pfam: Ribosomal protein S14p/S29e
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Chains: AO, CO
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ4 (Residues: 1-89; Coverage: 100%)
Gene names: JW3134, b3165, rpsO, secC
Pfam: Ribosomal protein S15
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Chains: AP, CP
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T3 (Residues: 1-82; Coverage: 100%)
Gene names: JW2590, b2609, rpsP
Pfam: Ribosomal protein S16
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Chains: AQ, CQ
Length: 83 amino acids
Theoretical weight: 9.59 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG63 (Residues: 2-84; Coverage: 99%)
Gene names: JW3273, b3311, neaA, rpsQ
Pfam: Ribosomal protein S17
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Chains: AR, CR
Length: 74 amino acids
Theoretical weight: 8.87 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T7 (Residues: 2-75; Coverage: 99%)
Gene names: JW4160, b4202, rpsR
Pfam: Ribosomal protein S18
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Chains: AS, CS
Length: 91 amino acids
Theoretical weight: 10.32 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U3 (Residues: 2-92; Coverage: 99%)
Gene names: JW3278, b3316, rpsS
Pfam: Ribosomal protein S19
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Chains: AT, CT
Length: 86 amino acids
Theoretical weight: 9.58 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U7 (Residues: 2-87; Coverage: 99%)
Gene names: JW0022, b0023, rpsT
Pfam: Ribosomal protein S20
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Chains: AU, CU
Length: 70 amino acids
Theoretical weight: 8.39 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P68679 (Residues: 2-71; Coverage: 99%)
Gene names: JW3037, b3065, rpsU
Pfam: Ribosomal protein S21
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Chains: BD, DD
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Pfam: Ribosomal protein L3
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Chains: BK, DK
Length: 123 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADY3 (Residues: 1-123; Coverage: 100%)
Gene names: JW3272, b3310, rplN
Pfam: Ribosomal protein L14p/L23e
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Chains: BP, DP
Length: 114 amino acids
Theoretical weight: 13.03 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7K6 (Residues: 2-115; Coverage: 99%)
Gene names: JW2587, b2606, rplS
Pfam: Ribosomal protein L19
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Chains: BE, DE
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60723 (Residues: 1-201; Coverage: 100%)
Gene names: JW3281, b3319, eryA, rplD
Pfam: Ribosomal protein L4/L1 family
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Chains: B0, D0
Length: 56 amino acids
Theoretical weight: 6.33 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7N4 (Residues: 2-57; Coverage: 98%)
Gene names: JW1075, b1089, rpmF
Pfam: Ribosomal L32p protein family
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Chains: B4, D4
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7Q6 (Residues: 1-38; Coverage: 100%)
Gene names: JW3261, b3299, rpmJ
Pfam: Ribosomal protein L36
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Chains: B2, D2
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7P5 (Residues: 1-46; Coverage: 100%)
Gene names: JW3680, b3703, rimA, rpmH, ssaF
Pfam: Ribosomal protein L34
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Chains: BX, DX
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M6 (Residues: 1-63; Coverage: 100%)
Gene names: JW3274, b3312, rpmC
Pfam: Ribosomal L29 protein
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Chains: BJ, DJ
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AA10 (Residues: 1-142; Coverage: 100%)
Gene names: JW3200, b3231, rplM
Pfam: Ribosomal protein L13
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Chains: BN, DN
Length: 127 amino acids
Theoretical weight: 14.39 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG44 (Residues: 1-127; Coverage: 100%)
Gene names: JW3256, b3294, rplQ
Pfam: Ribosomal protein L17
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Chains: BO, DO
Length: 117 amino acids
Theoretical weight: 12.79 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0C018 (Residues: 1-117; Coverage: 100%)
Gene names: JW3266, b3304, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
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Chains: BQ, DQ
Length: 117 amino acids
Theoretical weight: 13.4 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L3 (Residues: 2-118; Coverage: 99%)
Gene names: JW1706, b1716, pdzA, rplT
Pfam: Ribosomal protein L20
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Chains: BR, DR
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG48 (Residues: 1-103; Coverage: 100%)
Gene names: JW3153, b3186, rplU
Pfam: Ribosomal prokaryotic L21 protein
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Chains: BT, DT
Length: 100 amino acids
Theoretical weight: 11.22 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ0 (Residues: 1-100; Coverage: 100%)
Gene names: JW3280, b3318, rplW
Pfam: Ribosomal protein L23
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Chains: BW, DW
Length: 84 amino acids
Theoretical weight: 9.02 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L8 (Residues: 2-85; Coverage: 99%)
Gene names: JW3152, b3185, rpmA
Pfam: Ribosomal L27 protein
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Chains: B6, D6
Length: 185 amino acids
Theoretical weight: 21.03 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q9WX76 (Residues: 1-185; Coverage: 100%)
Gene names: TTHA0858, frr
Pfam: Ribosome recycling factor
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Name: 16S ribosomal RNA
Representative chains: AA, CA
Length: 1542 nucleotides
Theoretical weight: 499.69 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: BA, DA
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Rfam: 5S ribosomal RNA

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Name: 23S ribosomal RNA
Representative chains: BB, DB
Length: 2904 nucleotides
Theoretical weight: 941.61 KDa
Rfam: Bacterial large subunit ribosomal RNA

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