4v4n

Electron Microscopy
9Å resolution

Structure of the Methanococcus jannaschii ribosome-SecYEBeta channel complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 69-mer (preferred)
Entry contents:
63 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (67 distinct):
Protein translocase subunit SecE Chain: A7
Molecule details ›
Chain: A7
Length: 67 amino acids
Theoretical weight: 7.7 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57817 (Residues: 1-67; Coverage: 91%)
Gene names: MJ0371, secE
Preprotein translocase subunit SecG Chain: A8
Molecule details ›
Chain: A8
Length: 52 amino acids
Theoretical weight: 5.85 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60460 (Residues: 1-52; Coverage: 98%)
Gene names: MJ0912.1, secG
50S ribosomal protein L39E Chain: Af
Molecule details ›
Chain: Af
Length: 51 amino acids
Theoretical weight: 6.32 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L19E Chain: AQ
Molecule details ›
Chain: AQ
Length: 150 amino acids
Theoretical weight: 17.92 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L22P Chain: AS
Molecule details ›
Chain: AS
Length: 150 amino acids
Theoretical weight: 17.04 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L23P Chain: AT
Molecule details ›
Chain: AT
Length: 84 amino acids
Theoretical weight: 9.65 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L24P Chain: AU
Molecule details ›
Chain: AU
Length: 121 amino acids
Theoretical weight: 14.36 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L29P Chain: AW
Molecule details ›
Chain: AW
Length: 72 amino acids
Theoretical weight: 8.55 KDa
Source organism: Methanocaldococcus jannaschii
Protein translocase subunit SecY Chain: AX
Molecule details ›
Chain: AX
Length: 436 amino acids
Theoretical weight: 47.48 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q60175 (Residues: 1-436; Coverage: 100%)
Gene names: MJ0478, secY
30S ribosomal protein L7AE Chains: AG, B3
Molecule details ›
Chains: AG, B3
Length: 123 amino acids
Theoretical weight: 13.41 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S3AE Chain: BA
Molecule details ›
Chain: BA
Length: 190 amino acids
Theoretical weight: 22.15 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S2P Chain: BB
Molecule details ›
Chain: BB
Length: 202 amino acids
Theoretical weight: 23.04 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S3P Chain: BC
Molecule details ›
Chain: BC
Length: 186 amino acids
Theoretical weight: 20.74 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S4P Chain: BD
Molecule details ›
Chain: BD
Length: 172 amino acids
Theoretical weight: 20.41 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S4E Chain: BE
Molecule details ›
Chain: BE
Length: 241 amino acids
Theoretical weight: 27.99 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S5P Chain: BF
Molecule details ›
Chain: BF
Length: 217 amino acids
Theoretical weight: 24.45 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S6E Chain: BG
Molecule details ›
Chain: BG
Length: 125 amino acids
Theoretical weight: 14 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S7P Chain: BH
Molecule details ›
Chain: BH
Length: 215 amino acids
Theoretical weight: 24.7 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S8P Chain: BI
Molecule details ›
Chain: BI
Length: 129 amino acids
Theoretical weight: 14.55 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S8E Chain: BJ
Molecule details ›
Chain: BJ
Length: 127 amino acids
Theoretical weight: 14.29 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S9P Chain: BK
Molecule details ›
Chain: BK
Length: 135 amino acids
Theoretical weight: 15.32 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S10P Chain: BL
Molecule details ›
Chain: BL
Length: 102 amino acids
Theoretical weight: 11.77 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S11P Chain: BM
Molecule details ›
Chain: BM
Length: 133 amino acids
Theoretical weight: 14.28 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S12P Chain: BN
Molecule details ›
Chain: BN
Length: 145 amino acids
Theoretical weight: 16.25 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S13P Chain: BO
Molecule details ›
Chain: BO
Length: 148 amino acids
Theoretical weight: 16.95 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S14P Chain: BP
Molecule details ›
Chain: BP
Length: 56 amino acids
Theoretical weight: 6.63 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S15P/S13E Chain: BQ
Molecule details ›
Chain: BQ
Length: 158 amino acids
Theoretical weight: 18.64 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S17P Chain: BR
Molecule details ›
Chain: BR
Length: 113 amino acids
Theoretical weight: 13.3 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S17E Chain: BS
Molecule details ›
Chain: BS
Length: 67 amino acids
Theoretical weight: 7.94 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S19P Chain: BT
Molecule details ›
Chain: BT
Length: 111 amino acids
Theoretical weight: 13.18 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S19E Chain: BU
Molecule details ›
Chain: BU
Length: 144 amino acids
Theoretical weight: 16.63 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S24E Chain: BV
Molecule details ›
Chain: BV
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S27E Chain: BW
Molecule details ›
Chain: BW
Length: 63 amino acids
Theoretical weight: 6.89 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S28E Chain: BX
Molecule details ›
Chain: BX
Length: 71 amino acids
Theoretical weight: 8.12 KDa
Source organism: Methanocaldococcus jannaschii
30S ribosomal protein S27AE Chain: BY
Molecule details ›
Chain: BY
Length: 50 amino acids
Theoretical weight: 5.86 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L14E Chains: A5, AK
Molecule details ›
Chains: A5, AK
Length: 81 amino acids
Theoretical weight: 8.75 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L1P Chain: AA
Molecule details ›
Chain: AA
Length: 216 amino acids
Theoretical weight: 23.89 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L31E Chain: Aa
Molecule details ›
Chain: Aa
Length: 92 amino acids
Theoretical weight: 10.77 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L2 Chain: AB
Molecule details ›
Chain: AB
Length: 239 amino acids
Theoretical weight: 26.09 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L32E Chain: Ab
Molecule details ›
Chain: Ab
Length: 127 amino acids
Theoretical weight: 15.23 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L3P Chain: AC
Molecule details ›
Chain: AC
Length: 365 amino acids
Theoretical weight: 41.47 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L4P Chain: AD
Molecule details ›
Chain: AD
Length: 255 amino acids
Theoretical weight: 28.77 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L34E Chain: Ad
Molecule details ›
Chain: Ad
Length: 89 amino acids
Theoretical weight: 10.68 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L5P Chain: AE
Molecule details ›
Chain: AE
Length: 186 amino acids
Theoretical weight: 21.27 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L37E Chain: Ae
Molecule details ›
Chain: Ae
Length: 62 amino acids
Theoretical weight: 7.25 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L6P Chain: AF
Molecule details ›
Chain: AF
Length: 184 amino acids
Theoretical weight: 20.89 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L40E Chain: Ag
Molecule details ›
Chain: Ag
Length: 45 amino acids
Theoretical weight: 5.33 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L11P Chain: AH
Molecule details ›
Chain: AH
Length: 134 amino acids
Theoretical weight: 14.16 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L41E Chain: Ah
Molecule details ›
Chain: Ah
Length: 24 amino acids
Theoretical weight: 3.28 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L13P Chain: AI
Molecule details ›
Chain: AI
Length: 142 amino acids
Theoretical weight: 16.35 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L37AE Chain: Ai
Molecule details ›
Chain: Ai
Length: 78 amino acids
Theoretical weight: 8.45 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L14P Chain: AJ
Molecule details ›
Chain: AJ
Length: 132 amino acids
Theoretical weight: 14.45 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L44E Chain: Aj
Molecule details ›
Chain: Aj
Length: 94 amino acids
Theoretical weight: 11.23 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein P0/L10E Chain: Ak
Molecule details ›
Chain: Ak
Length: 212 amino acids
Theoretical weight: 23.26 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L15P Chain: AL
Molecule details ›
Chain: AL
Length: 147 amino acids
Theoretical weight: 16.44 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L15E Chain: AM
Molecule details ›
Chain: AM
Length: 194 amino acids
Theoretical weight: 22.64 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L10E/L16 Chain: AN
Molecule details ›
Chain: AN
Length: 168 amino acids
Theoretical weight: 19.58 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L18P Chain: AO
Molecule details ›
Chain: AO
Length: 197 amino acids
Theoretical weight: 22.63 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L18E Chain: AP
Molecule details ›
Chain: AP
Length: 120 amino acids
Theoretical weight: 13.76 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L21E Chain: AR
Molecule details ›
Chain: AR
Length: 95 amino acids
Theoretical weight: 11.14 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L24E Chain: AV
Molecule details ›
Chain: AV
Length: 66 amino acids
Theoretical weight: 7.94 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L30P Chain: AY
Molecule details ›
Chain: AY
Length: 155 amino acids
Theoretical weight: 17.75 KDa
Source organism: Methanocaldococcus jannaschii
50S ribosomal protein L30E Chain: AZ
Molecule details ›
Chain: AZ
Length: 99 amino acids
Theoretical weight: 10.73 KDa
Source organism: Methanocaldococcus jannaschii
E-tRNA Chain: B1
Molecule details ›
Chain: B1
Length: 77 nucleotides
Theoretical weight: 24.88 KDa
Sequence domains: tRNA
16S ribosomal RNA Chain: B2
Molecule details ›
Chain: B2
Length: 1495 nucleotides
Theoretical weight: 485.46 KDa
Sequence domains: Archaeal small subunit ribosomal RNA
23S ribosomal RNA Chain: A1
Molecule details ›
Chain: A1
Length: 3049 nucleotides
Theoretical weight: 990.82 KDa
Sequence domains: Archaeal large subunit ribosomal RNA
5S ribosomal RNA Chain: A3
Molecule details ›
Chain: A3
Length: 126 nucleotides
Theoretical weight: 40.69 KDa
Sequence domains: 5S ribosomal RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-5691
Expression system: Escherichia coli BL21(DE3)