› 4v3c

X-ray diffraction
1.84Å resolution

The structure of alpha2,3-sialyltransferase variant 2 from Pasteurella dagmatis in complex with the donor product CMP

Released:
DOI: 10.2210/pdb4v3c/pdb
PDB 4v3c coloured by chain and viewed from the front.
PDB 4v3c coloured by chain and viewed from the side.
PDB 4v3c coloured by chain and viewed from the top.
Source organism: Pasteurella dagmatis
Entry authors: Pavkov-Keller T, Schmoelzer K, Czabany T, Luley-Goedl C, Ribitsch D, Schwab H, Nidetzky B, Gruber K

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialyltransferase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 45.64 KDa
Source organism: Pasteurella dagmatis
Expression system: Escherichia coli
UniProt:
  • Canonical: K9UUI6 (Residues: 2-385; Coverage: 100%)
Sequence domains: Sialyltransferase PMO188
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand C 1 x C
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P65
Unit cell:
a: 111.75Å b: 111.75Å c: 65.19Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.173 0.218
Expression system: Escherichia coli

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Citations

6 review citations

Cellular and Molecular Engineering of Glycan Sialylation in Heterologous Systems.
Hombu et al. (2021)
5 more