› 4v2o

X-ray diffraction
2.13Å resolution

Structure of saposin B in complex with chloroquine

Released:
DOI: 10.2210/pdb4v2o/pdb
PDB 4v2o coloured by chain and viewed from the front.
PDB 4v2o coloured by chain and viewed from the side.
PDB 4v2o coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
The Lysosomal Protein Saposin���B Binds Chloroquine.
Huta BP, Mehlenbacher MR, Nie Y, Lai X, Zubieta C, Bou-Abdallah F, Doyle RP
ChemMedChem (2015)
PMID: 26616259

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Saposin-B Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 82 amino acids
Theoretical weight: 9.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07602 (Residues: 195-273; Coverage: 16%)
Gene names: GLBA, PSAP, SAP1
Sequence domains: Saposin-like type B, region 2
Structure domains: Saposin-like

Ligands and Environments

1 bound ligand:
Ligand CLQ 2 x CLQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3221
Unit cell:
a: 75.299Å b: 75.299Å c: 94.716Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.229 0.254
Expression system: Escherichia coli

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Citations

6 citation in other articles

Synergistic antiviral effect of hydroxychloroquine and azithromycin in combination against SARS-CoV-2: What molecular dynamics studies of virus-host interactions reveal.
Fantini et al. (2020)
5 more

10 mentions without citation

Structural and molecular modelling studies reveal a new mechanism of action of chloroquine and hydroxychloroquine against SARS-CoV-2 infection.
Fantini et al. (2020)
9 more