› 4v12

X-ray diffraction
1.5Å resolution

Crystal structure of the MSMEG_6754 dehydratase from Mycobacterium smegmatis

Released:
DOI: 10.2210/pdb4v12/pdb
PDB 4v12 coloured by chain and viewed from the front.
PDB 4v12 coloured by chain and viewed from the side.
PDB 4v12 coloured by chain and viewed from the top.
Source organism: Mycolicibacterium smegmatis
Primary publication:
A new dehydratase conferring innate resistance to thiacetazone and intra-amoebal survival of Mycobacterium smegmatis.
Carrère-Kremer S, Blaise M, Singh VK, Alibaud L, Tuaillon E, Halloum I, van de Weerd R, Guérardel Y, Drancourt M, Takiff H, Geurtsen J, Kremer L
Mol. Microbiol. (2015)
PMID: 25754266

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MaoC like domain protein Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 37.02 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0R724 (Residues: 1-342; Coverage: 100%)
Gene name: MSMEG_6754
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand POG 1 x POG
Ligand BTB 1 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P41212
Unit cell:
a: 84.39Å b: 84.39Å c: 122.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.159 0.178
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Pipeline of anti-Mycobacterium abscessus small molecules: Repurposable drugs and promising novel chemical entities.
Egorova et al. (2021)
2 more

1 mention without citation

Deletion of a dehydratase important for intracellular growth and cording renders rough Mycobacterium abscessus avirulent.
Halloum et al. (2016)