4v0g

X-ray diffraction
3Å resolution

JAK3 in complex with a covalent EGFR inhibitor

Released:
Source organism: Homo sapiens
Entry authors: Debreczeni JE, Hennessy EJ, Chuaquini C, Ashton S, Coclough N, Cross DAE, Eberlein C, Gingipalli L, Klinowska TCM, Orme JP, Sha L, Wu X

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase JAK3 Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 32.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52333 (Residues: 816-1098; Coverage: 25%)
Gene name: JAK3
Sequence domains: Protein tyrosine kinase
Structure domains:
Tyrosine-protein kinase JAK3 Chain: B
Molecule details ›
Chain: B
Length: 283 amino acids
Theoretical weight: 32.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52333 (Residues: 816-1098; Coverage: 25%)
Gene name: JAK3
Sequence domains: Protein tyrosine kinase
Structure domains:

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 57.14Å b: 99.032Å c: 111.373Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.252 0.295
Expression system: Escherichia coli