4uzz

X-ray diffraction
2.32Å resolution

Crystal structure of the TtIFT52-46 complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-125275 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Intraflagellar transport complex B protein 46 carboxy-terminal protein Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 13.46 KDa
Source organism: Tetrahymena thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q23KH7 (Residues: 221-332; Coverage: 33%)
Gene name: TTHERM_00193580
Sequence domains: Intraflagellar transport complex B protein 46 C terminal
Intraflagellar transporter-like protein Chain: B
Molecule details ›
Chain: B
Length: 68 amino acids
Theoretical weight: 7.71 KDa
Source organism: Tetrahymena thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I7LT74 (Residues: 371-434; Coverage: 15%)
Gene name: TTHERM_00648910
Sequence domains: Intraflagellar transport protein 52, C-terminal domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 84.394Å b: 84.394Å c: 95.477Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.239 0.264
Expression system: Escherichia coli BL21(DE3)