4uyk

X-ray diffraction
3.22Å resolution

Crystal structure of a Signal Recognition Particle Alu domain in the elongation arrest conformation

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Signal recognition particle 9 kDa protein Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 10.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49458 (Residues: 1-85; Coverage: 99%)
Gene name: SRP9
Sequence domains: Signal recognition particle 9 kDa protein (SRP9)
Structure domains: Signal recognition particle alu RNA binding heterodimer, srp9/1
Signal recognition particle 14 kDa protein Chain: B
Molecule details ›
Chain: B
Length: 107 amino acids
Theoretical weight: 12.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37108 (Residues: 1-107; Coverage: 79%)
Gene name: SRP14
Sequence domains: Signal recognition particle 14kD protein
Structure domains: Signal recognition particle alu RNA binding heterodimer, srp9/1
SRP RNA Chain: R
Molecule details ›
Chain: R
Length: 134 nucleotides
Theoretical weight: 43.52 KDa

Ligands and Environments

No bound ligands
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P43212
Unit cell:
a: 100.86Å b: 100.86Å c: 197.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.233
Expression system: Escherichia coli BL21(DE3)