4uy2

X-ray diffraction
2.7Å resolution

Crystal structure of the complex of the extracellular domain of human alpha9 nAChR with alpha-bungarotoxin.

Released:
Entry authors: Giastas P, Zouridakis M, Zarkadas E, Tzartos SJ

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Neuronal acetylcholine receptor subunit alpha-9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 218 amino acids
Theoretical weight: 25.31 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9UGM1 (Residues: 26-237; Coverage: 47%)
Gene names: CHRNA9, NACHRA9
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain
Alpha-bungarotoxin isoform V31 Chains: C, D
Molecule details ›
Chains: C, D
Length: 74 amino acids
Theoretical weight: 8.03 KDa
Source organism: Bungarus multicinctus
UniProt:
  • Canonical: P60616 (Residues: 22-95; Coverage: 100%)
Sequence domains: Snake toxin and toxin-like protein
Structure domains: CD59

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 42.319Å b: 119.348Å c: 79.107Å
α: 90° β: 103.02° γ: 90°
R-values:
R R work R free
0.262 0.258 0.331
Expression system: Komagataella pastoris