› 4uxt

Electron Microscopy
7.4Å resolution

Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins

Released:
DOI: 10.2210/pdb4uxt/pdb
PDB 4uxt coloured by chain and viewed from the front.
PDB 4uxt coloured by chain and viewed from the side.
PDB 4uxt coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins.
Atherton J, Farabella I, Yu IM, Rosenfeld SS, Houdusse A, Topf M, Moores CA
OpenAccess logo Elife e03680 (2014)
PMID: 25209998
Related structures: EMD-2769

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.11 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Kinesin heavy chain isoform 5A Chain: C
Molecule details ›
Chain: C
Length: 340 amino acids
Theoretical weight: 38.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12840 (Residues: 1-340; Coverage: 33%)
Gene names: KIF5A, NKHC1
Sequence domains: Kinesin motor domain

Ligands and Environments

5 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 1 x MG
Ligand GTP 1 x GTP
Ligand GDP 1 x GDP
Ligand TA1 1 x TA1
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.4Å
Relevant EMDB volumes: EMD-2769
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Emerging Insights into the Function of Kinesin-8 Proteins in Microtubule Length Regulation.
Shrestha et al. (2018)
8 more

2 mentions without citation

Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins.
Atherton et al. (2014)
1 more