› 4uxj

X-ray diffraction
3Å resolution

Leishmania major Thymidine Kinase in complex with dTTP

Released:
DOI: 10.2210/pdb4uxj/pdb
PDB 4uxj coloured by chain and viewed from the front.
PDB 4uxj coloured by chain and viewed from the side.
PDB 4uxj coloured by chain and viewed from the top.
Source organism: Leishmania major
Primary publication:
Structural and Kinetic Characterization of Thymidine Kinase from Leishmania major.
Timm J, Bosch-Navarrete C, Recio E, Nettleship JE, Rada H, González-Pacanowska D, Wilson KS
PLoS Negl Trop Dis 9 e0003781 (2015)
PMID: 25978379

Function and Biology Details

Reaction catalysed: 
ATP + thymidine = ADP + thymidine 5'-phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidine kinase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 191 amino acids
Theoretical weight: 21.98 KDa
Source organism: Leishmania major
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q4QC75 (Residues: 2-183; Coverage: 64%)
Gene name: LMJF_21_1210
Sequence domains: Thymidine kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 8 x ZN
Ligand TTP 8 x TTP
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 61.6Å b: 61.63Å c: 110.09Å
α: 81.07° β: 85.8° γ: 74.94°
R-values:
R R work R free
0.261 0.26 0.278
Expression system: Spodoptera frugiperda

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Citations

4 review citations

Alpha-Herpesvirus Thymidine Kinase Genes Mediate Viral Virulence and Are Potential Therapeutic Targets.
Xie et al. (2019)
3 more

2 mentions without citation

Structural and Kinetic Characterization of Thymidine Kinase from Leishmania major.
Timm et al. (2015)
1 more