X-ray diffraction
2.34Å resolution

Crystal structure of JNK1 bound to a MKK7 docking motif

Source organism: Homo sapiens
Primary publication:
Structure and dynamics of the MKK7-JNK signaling complex.
Proc. Natl. Acad. Sci. U.S.A. 112 3409-14 (2015)
PMID: 25737554

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitogen-activated protein kinase 8 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 364 amino acids
Theoretical weight: 42.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P45983 (Residues: 1-364; Coverage: 85%)
Gene names: JNK1, MAPK8, PRKM8, SAPK1, SAPK1C
Sequence domains: Protein kinase domain
Structure domains:
Dual specificity mitogen-activated protein kinase kinase 7 Chains: F, G, H, I
Molecule details ›
Chains: F, G, H, I
Length: 12 amino acids
Theoretical weight: 1.39 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: O14733 (Residues: 37-48; Coverage: 3%)
Gene names: JNKK2, MAP2K7, MEK7, MKK7, PRKMK7, SKK4

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 108.666Å b: 180.159Å c: 101.144Å
α: 90° β: 110.3° γ: 90°
R R work R free
0.202 0.2 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided