PDBe 4ux8

Electron Microscopy
24Å resolution

RET recognition of GDNF-GFRalpha1 ligand by a composite binding site promotes membrane-proximal self-association


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Extracellular cell-membrane anchored RET cadherin 120 kDa fragment Chains: A, B
Molecule details ›
Chains: A, B
Length: 607 amino acids
Theoretical weight: 67.92 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
  • Canonical: P07949 (Residues: 29-635; Coverage: 56%)
Gene names: CDHF12, CDHR16, PTC, RET, RET51
Sequence domains:
GDNF family receptor alpha-1 Chains: C, E
Molecule details ›
Chains: C, E
Length: 463 amino acids
Theoretical weight: 51.09 KDa
Source organism: Rattus norvegicus
Expression system: Cricetulus griseus
  • Canonical: Q62997 (Residues: 1-468; Coverage: 99%)
Gene names: Gdnfra, Gfra1, Retl1, Trnr1
Sequence domains: GDNF/GAS1 domain
Glial cell line-derived neurotrophic factor Chains: D, F
Molecule details ›
Chains: D, F
Length: 134 amino acids
Theoretical weight: 15.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P39905 (Residues: 78-211; Coverage: 70%)
Gene name: GDNF
Sequence domains: Transforming growth factor beta like domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 24Å
Relevant EMDB volumes: EMD-2712
Expression systems:
  • Cricetulus griseus
  • Escherichia coli