› 4uuy

X-ray diffraction
2.14Å resolution

Structural Identification of the Vps18 beta-propeller reveals a critical role in the HOPS complex stability and function.

Released:
DOI: 10.2210/pdb4uuy/pdb
PDB entry 4uuy coloured by chain, front view.
PDB entry 4uuy coloured by chain, side view.
PDB entry 4uuy coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural identification of the Vps18 β-propeller reveals a critical role in the HOPS complex stability and function.
Behrmann H, Lürick A, Kuhlee A, Balderhaar HK, Bröcker C, Kümmel D, Engelbrecht-Vandré S, Gohlke U, Raunser S, Heinemann U, Ungermann C
J Biol Chem 289 33503-12 (2014)
PMID: 25324549

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151076 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar membrane protein PEP3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 40.41 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27801 (Residues: 2-348; Coverage: 38%)
Gene names: L9634.2, PEP3, VAM8, VPS18, VPT18, YLR148W
Sequence domains: Pep3/Vps18/deep orange beta-propeller domain

Ligands and Environments

3 bound ligands:
Ligand SO4 6 x SO4
Ligand EDO 3 x EDO
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 56.25Å b: 77.534Å c: 84.508Å
α: 90° β: 98.91° γ: 90°
R-values:
R R work R free
0.204 0.201 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Multiple Roles of the Small GTPase Rab7.
Guerra et al. (2016)
2 more

1 mention without citation

Structural identification of the Vps18 β-propeller reveals a critical role in the HOPS complex stability and function.
Behrmann et al. (2014)