4uuv

X-ray diffraction
2.8Å resolution

Structure of the DNA binding ETS domain of human ETV4 in complex with DNA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
ETS translocation variant 4 Chains: A, D, G, J, M, P, S, V
Molecule details ›
Chains: A, D, G, J, M, P, S, V
Length: 100 amino acids
Theoretical weight: 11.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P43268 (Residues: 338-435; Coverage: 20%)
Gene names: E1AF, ETV4, PEA3
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*AP*CP*CP*GP*GP*AP*AP*GP*TP*GP)-3' Chains: B, E, H, K, N, Q, T, W
Molecule details ›
Chains: B, E, H, K, N, Q, T, W
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*AP*CP*TP*TP*CP*CP*GP*GP*TP*CP)-3' Chains: C, F, I, L, O, R, U
Molecule details ›
Chains: C, F, I, L, O, R, U
Length: 10 nucleotides
Theoretical weight: 3 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*AP*CP*TP*TP*CP*CP*GP*GP*TP*CP)-3' Chain: X
Molecule details ›
Chain: X
Length: 10 nucleotides
Theoretical weight: 3.04 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 176.651Å b: 46.133Å c: 171.15Å
α: 90° β: 96.69° γ: 90°
R-values:
R R work R free
0.205 0.203 0.248
Expression systems:
  • Escherichia coli BL21
  • Not provided