› 4uui

X-ray diffraction
1.79Å resolution

A case study for twinned data analysis: multiple crystal forms of the enzyme N-acetyl-neuraminic lyase

Released:
DOI: 10.2210/pdb4uui/pdb
PDB entry 4uui coloured by chain, front view.
PDB entry 4uui coloured by chain, side view.
PDB entry 4uui coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Campeotto I, Phillips SEV, Pearson AR

Function and Biology Details

Reaction catalysed: 
Aceneneuramate = N-acetyl-D-mannosamine + pyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylneuraminate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 304 amino acids
Theoretical weight: 33.62 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6L4 (Residues: 2-297; Coverage: 100%)
Gene names: JW3194, b3225, nanA, npl
Sequence domains: Dihydrodipicolinate synthetase family
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand PYR 4 x PYR
Ligand NA 1 x NA
Ligand 1PE 2 x 1PE
1 modified residue:
Ligand KPI 4 x KPI

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 77.985Å b: 116.665Å c: 83.678Å
α: 90° β: 118.06° γ: 90°
R-values:
R R work R free
0.157 0.155 0.184
Expression system: Escherichia coli BL21(DE3)

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