X-ray diffraction
3Å resolution

Crystal structure of zebrafish Sirtuin 5 in complex with 3-methyl- succinylated CPS1-peptide


Function and Biology Details

Reaction catalysed:
(1a) ATP + HCO(3)(-) = ADP + carboxyphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD-dependent protein deacylase sirtuin-5, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 275 amino acids
Theoretical weight: 30.42 KDa
Source organism: Danio rerio
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q6DHI5 (Residues: 30-298; Coverage: 88%)
Gene names: si:ch211-121a2.1, sirt5
Sequence domains: Sir2 family
Structure domains:
Carbamoyl-phosphate synthase [ammonia], mitochondrial Chain: D
Molecule details ›
Chain: D
Length: 9 amino acids
Theoretical weight: 969 Da
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P31327 (Residues: 524-531; Coverage: 1%)
Gene name: CPS1

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6522
Unit cell:
a: 87.51Å b: 87.51Å c: 314.38Å
α: 90° β: 90° γ: 120°
R R work R free
0.202 0.199 0.27
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided