› 4utu

X-ray diffraction
1.45Å resolution

Structural and biochemical characterization of the N- acetylmannosamine-6-phosphate 2-epimerase from Clostridium perfringens

Released:
DOI: 10.2210/pdb4utu/pdb
PDB 4utu coloured by chain and viewed from the front.
PDB 4utu coloured by chain and viewed from the side.
PDB 4utu coloured by chain and viewed from the top.
Source organism: Clostridium perfringens str. 13
Entry authors: Pelissier MC, Sebban-Kreuzer C, Guerlesquin F, Brannigan JA, Davies GJ, Bourne Y, Vincent F

Function and Biology Details

Reaction catalysed: 
N-acyl-D-glucosamine 6-phosphate = N-acyl-D-mannosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative N-acetylmannosamine-6-phosphate 2-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 25.06 KDa
Source organism: Clostridium perfringens str. 13
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8XNZ3 (Residues: 1-220; Coverage: 100%)
Gene names: CPE0184, nanE, nanP
Sequence domains: Putative N-acetylmannosamine-6-phosphate epimerase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand LRY 2 x LRY
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 36.714Å b: 82.147Å c: 75.195Å
α: 90° β: 92.48° γ: 90°
R-values:
R R work R free
0.141 0.14 0.169
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Racemases and epimerases operating through a 1,1-proton transfer mechanism: reactivity, mechanism and inhibition.
Lloyd et al. (2021)
5 other articles

1 mention without citation

Structural and functional characterization of the Clostridium perfringens N-acetylmannosamine-6-phosphate 2-epimerase essential for the sialic acid salvage pathway.
Pélissier et al. (2014)