4utl

X-ray diffraction
1.23Å resolution

XenA - reduced - Y183F variant in complex with coumarin

Released:

Function and Biology Details

Reaction catalysed:
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxidored_FMN domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 365 amino acids
Theoretical weight: 40.09 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3ZDM6 (Residues: 1-363; Coverage: 100%)
Gene name: xenA
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FNR 1 x FNR
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY
Spacegroup: I222
Unit cell:
a: 57.812Å b: 83.525Å c: 157.331Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.121 0.121 0.142
Expression system: Escherichia coli