4us1

X-ray diffraction
2.65Å resolution

The crystal structure of H-Ras and SOS in complex with ligands

Released:
Source organism: Homo sapiens
Entry authors: Winter JJG, Anderson M, Blades K, Brassington C, Breeze AL, Chresta C, Embrey K, Fairley G, Faulder P, Finlay MRV, Kettle JG, Nowak T, Overman R, Patel SJ, Perkins P, Spadola L, Tart J, Tucker J, Wrigley G

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTPase HRas, N-terminally processed Chain: R
Molecule details ›
Chain: R
Length: 185 amino acids
Theoretical weight: 21.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Son of sevenless homolog 1 Chain: S
Molecule details ›
Chain: S
Length: 487 amino acids
Theoretical weight: 57.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07889 (Residues: 564-1049; Coverage: 37%)
Gene name: SOS1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I422
Unit cell:
a: 149.56Å b: 149.56Å c: 199.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.231
Expression system: Escherichia coli