X-ray diffraction
3.8Å resolution

Crystal structure of the wild-type diacylglycerol kinase refolded in the lipid cubic phase


Function and Biology Details

Reaction catalysed:
ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Diacylglycerol kinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 130 amino acids
Theoretical weight: 14.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P0ABN1 (Residues: 2-122; Coverage: 99%)
Gene names: JW4002, b4042, dgkA
Sequence domains: Prokaryotic diacylglycerol kinase

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P3121
Unit cell:
a: 75.39Å b: 75.39Å c: 197.2Å
α: 90° β: 90° γ: 120°
R R work R free
0.326 0.324 0.372
Expression system: Escherichia coli