› 4up4

X-ray diffraction
1.95Å resolution

Structure of the recombinant lectin PVL from Psathyrella velutina in complex with GlcNAcb-D-1,3Galactoside

Released:
DOI: 10.2210/pdb4up4/pdb
PDB entry 4up4 coloured by chain, front view.
PDB entry 4up4 coloured by chain, side view.
PDB entry 4up4 coloured by chain, top view.
Source organism: Lacrymaria velutina
Primary publication:
A recombinant fungal lectin for labeling truncated glycans on human cancer cells.
Audfray A, Beldjoudi M, Breiman A, Hurbin A, Boos I, Unverzagt C, Bouras M, Lantuejoul S, Coll JL, Varrot A, Le Pendu J, Busser B, Imberty A
PLoS One 10 e0128190 (2015)
PMID: 26042789

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174236 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lectin PVL Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 43.03 KDa
Source organism: Lacrymaria velutina
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q309D1 (Residues: 1-395; Coverage: 100%)
Gene name: pvl
Sequence domains: FG-GAP-like repeat

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL
Carbohydrate polymer
3 bound ligands:
Ligand CA 6 x CA
Ligand NDG 2 x NDG
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 54.814Å b: 97.468Å c: 158.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies.
Sabotič et al. (2016)
2 more

1 mention without citation

A recombinant fungal lectin for labeling truncated glycans on human cancer cells.
Audfray et al. (2015)