X-ray diffraction
2.3Å resolution

Structure of bovine endothelial nitric oxide synthase heme domain in complex with N1-(5-(2-(6-Amino-4-methylpyridin-2-yl)ethyl)pyridin-3- yl)-N1,N2-dimethylethane-1,2-diamine


Function and Biology Details

Reaction catalysed:
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Nitric oxide synthase, endothelial Chains: A, B
Molecule details ›
Chains: A, B
Length: 443 amino acids
Theoretical weight: 49.73 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P29473 (Residues: 40-482; Coverage: 37%)
Gene name: NOS3
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments

Cofactor: Ligand HEM 2 x HEM
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 57.94Å b: 106.32Å c: 156.85Å
α: 90° β: 90° γ: 90°
R R work R free
0.164 0.161 0.217
Expression system: Escherichia coli BL21(DE3)