4uft Citations

Near-atomic cryo-EM structure of the helical measles virus nucleocapsid.


Measles is a highly contagious human disease. We used cryo-electron microscopy and single particle-based helical image analysis to determine the 4.3 Å resolution structure of the helical nucleocapsid formed by the folded domain of the Measles Virus nucleoprotein encapsidating an RNA. The resulting pseudoatomic model of the Measles Virus nucleocapsid offers important insights into the mechanism of the helical polymerisation of nucleocapsids of negative-strand RNA viruses, in particular via the exchange subdomains of the nucleoprotein.The structure reveals the mode of the nucleoprotein-RNA interaction and explains why each nucleoprotein of Measles Virus binds six nucleotides whereas the Respiratory Syncytial Virus nucleoprotein binds seven. It provides a rational basis for further analysis of Measles Virus replication and transcription, and reveals potential targets for drug design.

Reviews - 4uft mentioned but not cited (1)

  1. The Nucleoprotein and Phosphoprotein of Measles Virus. Guseva S, Milles S, Blackledge M, Ruigrok RWH. Front Microbiol 10 1832 (2019)

Articles - 4uft mentioned but not cited (2)

  1. Self-Assembly of Measles Virus Nucleocapsid-like Particles: Kinetics and RNA Sequence Dependence. Milles S, Jensen MR, Communie G, Maurin D, Schoehn G, Ruigrok RW, Blackledge M. Angew. Chem. Int. Ed. Engl. 55 9356-9360 (2016)
  2. Assembly and cryo-EM structures of RNA-specific measles virus nucleocapsids provide mechanistic insight into paramyxoviral replication. Desfosses A, Milles S, Jensen MR, Guseva S, Colletier JP, Maurin D, Schoehn G, Gutsche I, Ruigrok RWH, Blackledge M. Proc. Natl. Acad. Sci. U.S.A. (2019)

Reviews citing this publication (22)

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Articles citing this publication (37)

  1. Crystal Structure of the Measles Virus Nucleoprotein Core in Complex with an N-Terminal Region of Phosphoprotein. Guryanov SG, Liljeroos L, Kasaragod P, Kajander T, Butcher SJ. J. Virol. 90 2849-2857 (2016)
  2. A Druggable Pocket at the Nucleocapsid/Phosphoprotein Interaction Site of Human Respiratory Syncytial Virus. Ouizougun-Oubari M, Pereira N, Tarus B, Galloux M, Lassoued S, Fix J, Tortorici MA, Hoos S, Baron B, England P, Desmaële D, Couvreur P, Bontems F, Rey FA, Eléouët JF, Sizun C, Slama-Schwok A, Duquerroy S. J. Virol. 89 11129-11143 (2015)
  3. Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly. Guseva S, Milles S, Jensen MR, Salvi N, Kleman JP, Maurin D, Ruigrok RWH, Blackledge M. Sci Adv 6 eaaz7095 (2020)
  4. Nucleocapsid assembly in pneumoviruses is regulated by conformational switching of the N protein. Renner M, Bertinelli M, Leyrat C, Paesen GC, Saraiva de Oliveira LF, Huiskonen JT, Grimes JM. Elife 5 e12627 (2016)
  5. Modulation of Re-initiation of Measles Virus Transcription at Intergenic Regions by PXD to NTAIL Binding Strength. Bloyet LM, Brunel J, Dosnon M, Hamon V, Erales J, Gruet A, Lazert C, Bignon C, Roche P, Longhi S, Gerlier D. PLoS Pathog. 12 e1006058 (2016)
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  8. Cryo-EM structure of the Ebola virus nucleoprotein-RNA complex at 3.6 Å resolution. Sugita Y, Matsunami H, Kawaoka Y, Noda T, Wolf M. Nature 563 137-140 (2018)
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  13. An ultraweak interaction in the intrinsically disordered replication machinery is essential for measles virus function. Milles S, Jensen MR, Lazert C, Guseva S, Ivashchenko S, Communie G, Maurin D, Gerlier D, Ruigrok RWH, Blackledge M. Sci Adv 4 eaat7778 (2018)
  14. Binding of RNA by the Nucleoproteins of Influenza Viruses A and B. Labaronne A, Swale C, Monod A, Schoehn G, Crépin T, Ruigrok RW. Viruses 8 (2016)
  15. Releasing the Genomic RNA Sequestered in the Mumps Virus Nucleocapsid. Severin C, Terrell JR, Zengel JR, Cox R, Plemper RK, He B, Luo M. J. Virol. 90 10113-10119 (2016)
  16. Structural characterization by transmission electron microscopy and immunoreactivity of recombinant Hendra virus nucleocapsid protein expressed and purified from Escherichia coli. Pearce LA, Yu M, Waddington LJ, Barr JA, Scoble JA, Crameri GS, McKinstry WJ. Protein Expr Purif 116 19-29 (2015)
  17. Synthesis of human parainfluenza virus 2 nucleocapsid protein in yeast as nucleocapsid-like particles and investigation of its antigenic structure. Bulavaitė A, Lasickienė R, Vaitiekaitė A, Sasnauskas K, Žvirblienė A. Appl. Microbiol. Biotechnol. 100 4523-4534 (2016)
  18. The Unstructured Paramyxovirus Nucleocapsid Protein Tail Domain Modulates Viral Pathogenesis through Regulation of Transcriptase Activity. Thakkar VD, Cox RM, Sawatsky B, da Fontoura Budaszewski R, Sourimant J, Wabbel K, Makhsous N, Greninger AL, von Messling V, Plemper RK. J. Virol. 92 (2018)
  19. The glutamic residue at position 402 in the C-terminus of Newcastle disease virus nucleoprotein is critical for the virus. Yu X, Cheng J, He Z, Li C, Song Y, Xue J, Yang H, Zhang R, Zhang G. Sci Rep 7 17471 (2017)
  20. A Polyamide Inhibits Replication of Vesicular Stomatitis Virus by Targeting RNA in the Nucleocapsid. Gumpper RH, Li W, Castañeda CH, Scuderi MJ, Bashkin JK, Luo M. J. Virol. 92 (2018)
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  26. Bipartite interface of the measles virus phosphoprotein X domain with the large polymerase protein regulates viral polymerase dynamics. Du Pont V, Jiang Y, Plemper RK. PLoS Pathog. 15 e1007995 (2019)
  27. Complementary Mutations in the N and L Proteins for Restoration of Viral RNA Synthesis. Li W, Gumpper RH, Uddin Y, Schmidt-Krey I, Luo M. J. Virol. 92 (2018)
  28. High resolution cryo-EM structure of the helical RNA-bound Hantaan virus nucleocapsid reveals its assembly mechanisms. Arragain B, Reguera J, Desfosses A, Gutsche I, Schoehn G, Malet H. Elife 8 (2019)
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