4ufo

X-ray diffraction
2.02Å resolution

Laboratory evolved variant R-C1B1D33E6 of potato epoxide hydrolase StEH1

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 37.07 KDa
Source organism: Solanum tuberosum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q41415 (Residues: 1-321; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 55.678Å b: 98.444Å c: 122.571Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.232
Expression system: Escherichia coli