4uf5

X-ray diffraction
3.7Å resolution

Crystal structure of UCH-L5 in complex with inhibitory fragment of INO80G

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin carboxyl-terminal hydrolase isozyme L5 Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5K5 (Residues: 1-329; Coverage: 100%)
Gene names: AD-019, CGI-70, UCH37, UCHL5
Sequence domains:
Nuclear factor related to kappa-B-binding protein Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 15.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6P4R8 (Residues: 40-170; Coverage: 10%)
Gene names: INO80G, NFRKB

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P4122
Unit cell:
a: 95.302Å b: 95.302Å c: 132.576Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.312 0.307 0.357
Expression system: Escherichia coli BL21(DE3)