› 4ueo

X-ray diffraction
2Å resolution

Open state of galactitol-1-phosphate 5-dehydrogenase from E. coli, with zinc in the catalytic site.

Released:
DOI: 10.2210/pdb4ueo/pdb
PDB entry 4ueo coloured by chain, front view.
PDB entry 4ueo coloured by chain, side view.
PDB entry 4ueo coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Enantioselective oxidation of galactitol 1-phosphate by galactitol-1-phosphate 5-dehydrogenase from Escherichia coli.
Benavente R, Esteban-Torres M, Kohring GW, Cortés-Cabrera Á, Sánchez-Murcia PA, Gago F, Acebrón I, de las Rivas B, Muñoz R, Mancheño JM
Acta Crystallogr D Biol Crystallogr 71 1540-54 (2015)
PMID: 26143925

Function and Biology Details

Reaction catalysed: 
Galactitol 1-phosphate + NAD(+) = D-tagatose 6-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141923 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactitol 1-phosphate 5-dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 346 amino acids
Theoretical weight: 37.43 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A9S3 (Residues: 1-346; Coverage: 100%)
Gene names: JW2075, b2091, gatD
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P21
Unit cell:
a: 43.306Å b: 76.906Å c: 108.651Å
α: 90° β: 95.51° γ: 90°
R-values:
R R work R free
0.198 0.194 0.256
Expression system: Escherichia coli BL21

Quick links

Citations

3 citation in other articles

Dynamic Behavior of Trigger Factor on the Ribosome.
Deeng et al. (2016)
2 more