› 4udo

X-ray diffraction
2.22Å resolution

structure of Mn-bound periplasmic metal binding protein from candidatus liberibacter asiaticus

Released:
DOI: 10.2210/pdb4udo/pdb
PDB entry 4udo coloured by chain, front view.
PDB entry 4udo coloured by chain, side view.
PDB entry 4udo coloured by chain, top view.
Source organism: Candidatus Liberibacter asiaticus
Primary publication:
Crystal structure of a periplasmic solute binding protein in metal-free, intermediate and metal-bound states from Candidatus Liberibacter asiaticus.
Sharma N, Selvakumar P, Bhose S, Ghosh DK, Kumar P, Sharma AK
J Struct Biol 189 184-94 (2015)
PMID: 25641618

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111644 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic solute binding protein Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 30.9 KDa
Source organism: Candidatus Liberibacter asiaticus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: C6XF58 (Residues: 20-294; Coverage: 100%)
Gene name: CLIBASIA_02120
Sequence domains: Zinc-uptake complex component A periplasmic
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

4 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 2 x GOL
Ligand MN 1 x MN
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P3221
Unit cell:
a: 94.32Å b: 94.32Å c: 94.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.165 0.164 0.196
Expression system: Escherichia coli BL21

Quick links

Citations

5 citation in other articles

Mechanisms of zinc binding to the solute-binding protein AztC and transfer from the metallochaperone AztD.
Neupane et al. (2017)
4 more

2 mentions without citation

Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies.
Ghosh et al. (2022)
1 more