› 4ucz

X-ray diffraction
2.99Å resolution

X-ray structure and activities of an essential Mononegavirales L- protein domain

Released:
DOI: 10.2210/pdb4ucz/pdb
PDB 4ucz coloured by chain and viewed from the front.
PDB 4ucz coloured by chain and viewed from the side.
PDB 4ucz coloured by chain and viewed from the top.
Source organism: Human metapneumovirus
Primary publication:
X-ray structure and activities of an essential Mononegavirales L-protein domain.
Paesen GC, Collet A, Sallamand C, Debart F, Vasseur JJ, Canard B, Decroly E, Grimes JM
Nat Commun 6 8749 (2015)
PMID: 26549102

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
(1a) S-adenosyl-L-methionine + G(5')pppAACA-[mRNA] = S-adenosyl-L-homocysteine + G(5')pppAmACA-[mRNA]
(1a) (5')pppAACA-[mRNA] + (protein L)-L-histidine = diphosphate + (protein L)-L-histidyl-(5')phosphonato-AACA-[mRNA] + H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replicase Chains: A, B
Molecule details ›
Chains: A, B
Length: 415 amino acids
Theoretical weight: 47.66 KDa
Source organism: Human metapneumovirus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q91L20 (Residues: 1600-2005; Coverage: 20%)
Gene name: L

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand GTP 1 x GTP
Ligand GMP 1 x GMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 78.8Å b: 83.71Å c: 182.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.238
Expression system: Spodoptera frugiperda

Quick links

Citations

8 review citations

Structures of the Mononegavirales Polymerases.
Liang B. (2020)
7 more

3 mentions without citation

X-ray structure and activities of an essential Mononegavirales L-protein domain.
Paesen et al. (2015)
2 more