4uck

X-ray diffraction
2.66Å resolution

X-ray structure and activities of an essential Mononegavirales L- protein domain

Released:
Source organism: Human metapneumovirus
Entry authors: Paesen GC, Collet A, Sallamand C, Debart F, Vasseur JJ, Canard B, Decroly E, Grimes JM

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
(1a) S-adenosyl-L-methionine + G(5')pppAACA-[mRNA] = S-adenosyl-L-homocysteine + G(5')pppAmACA-[mRNA]
(1a) (5')pppAACA-[mRNA] + (protein L)-L-histidine = diphosphate + (protein L)-L-histidyl-(5')phosphonato-AACA-[mRNA] + H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replicase Chains: A, B
Molecule details ›
Chains: A, B
Length: 415 amino acids
Theoretical weight: 47.66 KDa
Source organism: Human metapneumovirus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q91L20 (Residues: 1600-2005; Coverage: 20%)
Gene name: L

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 80.34Å b: 84.35Å c: 181.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.204
Expression system: Spodoptera frugiperda