X-ray diffraction
2.4Å resolution

Crystal Structure Of a purine nucleoside phosphorylase (PSI-NYSGRC-029736) from Agrobacterium vitis

Source organism: Agrobacterium vitis
Entry authors: Cameron SA, Sampathkumar P, Ramagopal UA, Attonito J, Ahmed M, Bhosle R, Bonanno J, Chamala S, Chowdhury S, Glenn AS, Hammonds J, Hillerich B, Love JD, Seidel R, Stead M, Toro R, Wasserman SR, Schramm VL, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed:
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 31.33 KDa
Source organism: Agrobacterium vitis
Expression system: Escherichia coli BL21(DE3)
  • Canonical: B9JYS2 (Residues: 1-269; Coverage: 100%)
Gene name: Avi_0263
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P63
Unit cell:
a: 97.881Å b: 97.881Å c: 46.356Å
α: 90° β: 90° γ: 120°
R R work R free
0.182 0.179 0.224
Expression system: Escherichia coli BL21(DE3)