X-ray diffraction
1.95Å resolution

Structure of the 3-ketoacyl-CoA thiolase FadA5 from M. tuberculosis with an partially acetylated cysteine in complex with acetyl-CoA and CoA


Function and Biology Details

Reaction catalysed:
(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Steroid 3-ketoacyl-CoA thiolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 399 amino acids
Theoretical weight: 42.39 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Mycolicibacterium smegmatis MC2 155
  • Canonical: I6XHI4 (Residues: 1-391; Coverage: 100%)
Gene names: LH57_19345, Rv3546, fadA5
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

Cofactor: Ligand COA 2 x COA

Cofactor: Ligand ACO 1 x ACO
3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43212
Unit cell:
a: 124.68Å b: 124.68Å c: 124.71Å
α: 90° β: 90° γ: 90°
R R work R free
0.195 0.193 0.231
Expression system: Mycolicibacterium smegmatis MC2 155